Coutinho Hdm, Matias Eff, Santos Kka, Tintino S R, Souza Ces, Guedes Gmm, Santos Fad, Costa Jgm, Falcão-Silva Vs, Siqueira-Júnior Jp
Laboratory of Microbiology and Molecular Biology and Center of Exacts and Natural Sciences, Federal University of Paraba, Joo Pessoa (PB).
J Young Pharm. 2010 Oct;2(4):362-4. doi: 10.4103/0975-1483.71625.
This is the first on the modulation of norfloxacin antibiotic activity by the volatile compounds of an essential oil. We report the chemical composition and antibiotic modifying activity of the essential oil extracted from the leaves of Croton zehntneri Pax et Hoffm (variety estragole), using the minimal inhibitory dose method and gaseous contact. The leaves of Croton zehntneri Pax et Hoffm (Euphorbiaceae) were subjected to hydrodistillation, and the essential oil extracted was examined with respect to the chemical composition, by gas chromatography-mass spectrometry (GC/MS), and to inhibitory activity of efflux pump by gaseous contact. The main component of the essential oil of C. zehntneri was estragole (76,8%). The gaseous components of the oil enhanced the inhibition zone of norfloxacin in 39,5%. This result shows that this oil influences the antibiotic activity of norfloxacin, possibly affecting the bacterial NorA efflux system, and may be used as an adjuvant in the antibiotic therapy of multidrug resistant pathogens.
这是关于精油挥发性化合物对诺氟沙星抗生素活性调节作用的首次研究。我们采用最低抑菌剂量法和气态接触法，报告了从巴豆属植物泽恩特纳氏巴豆（变种为草蒿脑）叶片中提取的精油的化学成分及抗生素修饰活性。对大戟科泽恩特纳氏巴豆的叶片进行水蒸馏，通过气相色谱 - 质谱联用仪（GC/MS）分析提取的精油的化学成分，并通过气态接触法检测其对流出泵的抑制活性。泽恩特纳氏巴豆精油的主要成分是草蒿脑（76.8%）。该精油的气态成分使诺氟沙星的抑菌圈增大了39.5%。这一结果表明，这种精油会影响诺氟沙星的抗生素活性，可能作用于细菌的NorA流出系统，可作为多药耐药病原体抗生素治疗的佐剂。
