Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Int J Pharm. 2011 Apr 4;407(1-2):95-104. doi: 10.1016/j.ijpharm.2011.01.034. Epub 2011 Jan 25.
Microdermabrasion has been shown to increase skin permeability for transdermal drug delivery by damaging or removing skin's outer layer, stratum corneum. However, relationships between microdermabrasion parameters and effects on the stratum corneum barrier have not been developed. In this study, we determined the effect of microdermabrasion crystal flow rate, time, and suction pressure applied in both static and dynamic modes on the extent of stratum corneum removal from excised porcine skin. In addition to controlling the depth of tissue removal by microdermabrasion parameters, we also controlled the area of tissue removal by applying a metal mask patterned with 125- or 250-μm holes to selectively expose small spots of tissue to microdermabrasion. We found that the extent of stratum corneum removal depended strongly on the crystal flow rate and exposure time and only weakly on pressure or static/dynamic mode operation. Masking the skin was effective to localize stratum corneum removal to exposed sites. Overall, this study demonstrates that optimized microdermabrasion in combination with a mask can be used to selectively remove stratum corneum with three-dimensional control, which is important to translating this technique into a novel method of transdermal drug delivery.
微晶磨皮术通过破坏或去除皮肤的外层——角质层，来增加经皮药物递送的皮肤通透性。然而，微晶磨皮术参数与角质层屏障效应之间的关系尚未得到发展。在这项研究中，我们确定了在静态和动态模式下，微晶磨皮术的晶流率、时间和吸力压力对从猪皮上除去的角质层的程度的影响。除了通过微晶磨皮术参数控制组织去除的深度外，我们还通过应用带有 125 或 250μm 孔的金属掩模来控制组织去除的区域，从而有选择地将小面积的组织暴露于微晶磨皮术之下。我们发现，角质层去除的程度强烈依赖于晶体流速和暴露时间，而仅对压力或静态/动态模式操作的依赖性较弱。对皮肤进行遮蔽可以有效地将角质层去除局限于暴露部位。总的来说，这项研究表明，优化的微晶磨皮术与掩模结合使用，可以实现具有三维控制的角质层的选择性去除，这对于将该技术转化为一种新的经皮药物递送方法非常重要。
