Tsinghua University, Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Shenzhen, 518055, China.
J Biomed Opt. 2011 Jan-Feb;16(1):016003. doi: 10.1117/1.3528593.
Photodynamic therapy is a promising cancer treatment that involves activation of photosensitizer by visible light to create singlet oxygen. This highly reactive oxygen species is believed to induce cell death and tissue destruction in PDT. Our approach used a near-infrared area CCD with high quantum efficiency to detect singlet oxygen by its 1270-nm luminescence. Two-dimensional singlet oxygen images with its near-infrared luminescence during photosensitization could be obtained with a CCD integration time of 1 s, without scanning. Thus this system can produce singlet oxygen luminescence images faster and achieve more accurate measurements in comparison to raster-scanning methods. The experimental data show a linear relationship between the singlet oxygen luminescence intensity and sample concentration. This method provides a detection sensitivity of 0.0181 μg∕ml (benzoporphyrin derivative monoacid ring A dissolved in ethanol) and a spatial resolution better than 50 μm. A pilot study was conducted on a total of six female Kunming mice. The results from this study demonstrate the system's potential for in vivo measurements. Further experiments were carried out on two tumor-bearing nude mice. Singlet oxygen luminescence images were acquired from the tumor-bearing nude mouse with intravenous injection of BPD-MA, and the experimental results showed real-time singlet oxygen signal depletion as a function of the light exposure.
光动力疗法是一种很有前途的癌症治疗方法，它涉及通过可见光激活光敏剂以产生单线态氧。这种高反应性的氧物质被认为会在 PDT 中诱导细胞死亡和组织破坏。我们的方法使用了具有高量子效率的近红外区域 CCD 通过其 1270nm 的发光来检测单线态氧。通过使用 CCD 积分时间为 1 秒而无需扫描，就可以获得光敏化期间的二维单线态氧图像及其近红外发光。与光栅扫描方法相比，该系统可以更快地产生单线态氧发光图像并实现更准确的测量。实验数据表明，单线态氧发光强度与样品浓度之间存在线性关系。该方法提供了 0.0181μg/ml（溶于乙醇的苯并卟啉衍生物单酸环 A）的检测灵敏度和优于 50μm 的空间分辨率。对总共六只昆明雌性小鼠进行了一项初步研究。该研究结果表明了该系统在体内测量方面的潜力。在两只荷瘤裸鼠上进行了进一步的实验。通过静脉注射 BPD-MA 从荷瘤裸鼠上获得单线态氧发光图像，实验结果显示作为光暴露函数的实时单线态氧信号耗散。
