富 13C 的玻璃基质对 [1-13C]丙酮酸的动态核极化的影响。
The effect of 13C enrichment in the glassing matrix on dynamic nuclear polarization of [1-13C]pyruvate.
机构信息
Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
出版信息
Phys Med Biol. 2011 Mar 7;56(5):N85-92. doi: 10.1088/0031-9155/56/5/N01. Epub 2011 Feb 1.
Abstract
Dimethyl sulfoxide (DMSO) can effectively form a glassy matrix necessary for dynamic nuclear polarization (DNP) experiments. We tested the effects of (13)C enrichment in DMSO on DNP of [1-(13)C]pyruvate doped with trityl radical OX063Me. We found that the polarization build-up time τ of pyruvate in (13)C-labeled DMSO glassing solution is twice as fast as the unenriched DMSO while the nuclear magnetic resonance enhancement was unchanged. This indicates that (13)C-(13)C spin diffusion is a limiting factor in the kinetics of DNP in this system, but it has a minimal effect on the absolute value of polarization achievable for the target.
摘要
二甲基亚砜 (DMSO) 可以有效地形成用于动态核极化 (DNP) 实验的玻璃基质。我们测试了 DMSO 中 (13)C 富集对三苯基自由基 OX063Me 掺杂的 [1-(13)C]丙酮酸的 DNP 的影响。我们发现，(13)C 标记 DMSO 玻璃化溶液中丙酮酸的极化建立时间 τ 是未富集 DMSO 的两倍快，而核磁共振增强保持不变。这表明在该体系中，(13)C-(13)C 自旋扩散是 DNP 动力学的限制因素，但对目标可实现极化的绝对值影响最小。
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