Lama Bimala, Collins James H P, Downes Daniel, Smith Adam N, Long Joanna R
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, USA.
Department of Chemistry, University of Florida, Gainesville, FL, USA.
NMR Biomed. 2016 Mar;29(3):226-31. doi: 10.1002/nbm.3473. Epub 2016 Jan 14.
The hyperpolarization of metabolic substrates at low temperature using dynamic nuclear polarization (DNP), followed by rapid dissolution and injection into an MRSI or NMR system, allows in vitro or in vivo observation and tracking of biochemical reactions and metabolites in real time. This article describes an elegant approach to sample preparation which is broadly applicable for the rapid polarization of aqueous small-molecule substrate solutions and obviates the need for glassing agents. We demonstrate its utility for solutions of sodium acetate, pyruvate and butyrate. The polarization behavior of substrates prepared using rapid freezing without glassing agents enabled a 1.5-3-fold time savings in polarization buildup, whilst removing the need for toxic glassing agents used as standard for dissolution DNP. The achievable polarization with fully aqueous substrate solutions was equal to that observed using standard approaches and glassing agents. Copyright © 2016 John Wiley & Sons, Ltd.
利用动态核极化（DNP）在低温下对代谢底物进行超极化，随后快速溶解并注入磁共振波谱成像（MRSI）或核磁共振（NMR）系统，可实现对体外或体内生化反应和代谢物的实时观察与追踪。本文介绍了一种精妙的样品制备方法，该方法广泛适用于水性小分子底物溶液的快速极化，且无需使用玻璃化剂。我们展示了其在醋酸钠、丙酮酸和丁酸盐溶液中的效用。使用无玻璃化剂的快速冷冻法制备的底物的极化行为，在极化建立过程中节省了1.5至3倍的时间，同时无需使用用作溶解DNP标准的有毒玻璃化剂。完全水性底物溶液可实现的极化与使用标准方法和玻璃化剂所观察到的极化相当。版权所有© 2016约翰威立父子有限公司。
