Miao Qiang, Sun Jin-Long, Huang Fei, Wang Jing, Wang Pei, Zheng Ya-Fei, Wang Feng, Ma Chu-Fan
Department of Stomatology, Sixth Medical Center of PLA General Hospital, Beijing, China.
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
Front Bioeng Biotechnol. 2021 Sep 27;9:735889. doi: 10.3389/fbioe.2021.735889. eCollection 2021.
Percutaneous implants are widely used in clinical practice. However, infection is the main clinical problem of percutaneous implants. Titanium dioxide nanotubes are suitable for forming coatings on complex surfaces such as implants. HHC-36, a cationic antimicrobial peptide, has been identified to have a strong broad-spectrum antibacterial effect. In the present study, we use poly D,L-lactic acid (PDLLA) and poly lactic-co-glycolic acid (PLGA) coating to build HHC-36 sustained-release system on the surface of titanium dioxide nanotubes. The titanium specimens were anodized coated with HHC-36-PDLLA/PLGA. The morphology and surface elemental distribution of the specimens were evaluated. Besides, results in the present study demonstrated that with antibacterial peptide HHC-36 sustained-release coating, titanium dioxide nanotubes maintain effective drug release for 15 days , and show significant antibacterial activity. The proliferation of can be effectively inhibited by PDLLA/PLGA-HHC-36 coated titanium dioxide nanotube. In addition, PDLLA-HHC-36 and PLGA-HHC-36 coating was demonstrated to be biocompatible and antibacterial . These findings demonstrated that HHC-36 coated titanium nanotube could improve antibacterial potential of percutaneous implants, and indicated a novel and efficient strategy in preventing bacterial infection of percutaneous implants.
经皮植入物在临床实践中广泛应用。然而，感染是经皮植入物的主要临床问题。二氧化钛纳米管适用于在诸如植入物等复杂表面形成涂层。阳离子抗菌肽HHC - 36已被证实具有强大的广谱抗菌作用。在本研究中，我们使用聚D，L - 乳酸（PDLLA）和聚乳酸 - 乙醇酸共聚物（PLGA）涂层在二氧化钛纳米管表面构建HHC - 36缓释系统。将钛标本进行阳极氧化并用HHC - 36 - PDLLA/PLGA涂层。评估了标本的形态和表面元素分布。此外，本研究结果表明，带有抗菌肽HHC - 36缓释涂层的二氧化钛纳米管可维持15天的有效药物释放，并显示出显著的抗菌活性。PDLLA/PLGA - HHC - 36涂层的二氧化钛纳米管可有效抑制[此处原文缺失具体细胞或物质名称]的增殖。此外，PDLLA - HHC - 36和PLGA - HHC - 36涂层被证明具有生物相容性且抗菌。这些发现表明，HHC - 36涂层的钛纳米管可提高经皮植入物的抗菌潜力，并为预防经皮植入物的细菌感染指明了一种新颖且有效的策略。
