基于重复序列 PCR 的菌斑变形链球菌遗传多样性研究。
Genetic diversity of plaque mutans streptococci with rep-PCR.
机构信息
Department of Pediatric Dentistry, University of Alabama School of Dentistry, 1919 7th Avenue South, Birmingham, AL 35294, USA.
出版信息
J Dent Res. 2011 Mar;90(3):331-5. doi: 10.1177/0022034510386375.
Abstract
Mutans streptococci (MS) are key organisms associated with the etiology of dental caries. Using probabilities that were tested by oversampling, we designed this study to determine the minimal number of MS isolates from an individual required to evaluate diversity of genotypes. MS isolates were genotyped by repetitive extragenic palindromic-polymerase chain-reaction (rep-PCR). Analysis of 20 isolates from individuals resulted in a mean of 1.6 and 2.4 genotypes in children (N = 12) and adults (N = 10), respectively. In a follow-up study, reducing the number of isolates to 7-10 resulted in a theoretical probability of up to 78% for detecting up to 4 genotypes. A mean of 1.5 genotypes was found in 35 children and 10 adults. These findings provide evidence for the design of studies of MS genotyping that can serve as a model for the analysis of genotypes within individuals.
摘要
变形链球菌（MS）是与龋齿病因学相关的关键生物体。通过过采样测试概率，我们设计了这项研究，以确定从个体中评估基因型多样性所需的最少 MS 分离物数量。MS 分离物通过重复外回文聚合酶链反应（rep-PCR）进行基因分型。对来自个体的 20 个分离物的分析分别导致儿童（N=12）和成人（N=10）中分别有 1.6 和 2.4 种基因型。在后续研究中，将分离物数量减少到 7-10 个，可检测到 4 种基因型的理论概率高达 78%。在 35 名儿童和 10 名成人中发现平均有 1.5 种基因型。这些发现为 MS 基因分型研究的设计提供了证据，可以作为个体内基因型分析的模型。
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2
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Pediatr Dent. 2009 May-Jun;31(3):193-201.
3
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6
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7
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