Ogawa H, Tanaka H
Gan. 1978 Aug;69(4):539-44.
Acetone-fixed smears of DBA/2 mouse leukemia cells that produce clusters of intracytoplasmic A-particles (pronucleocapsids of mouse mammary tumor virus) were employed as an indirect immunofluorescence system to detect the antibody to A-particles in human sera. With positive test sera, specific fluorescence was easily detectable as discrete cytoplasmic granules at the site of A-particle clusters. The antibody was found in 26 (60%) out of 43 breast cancer patient sera and 4 (25%) of 16 mammary fibroadenoma patient sera, while only 4 (11%) out of 37 control woman sera were antibody-positive. In the case of breast cancer patients, occurrence of tha antibody was not specifically related to a particular type of tumor histology. In a considerable number of positive cases, the antibody tended to disappear within various lengths of time after surgical operation of the breast cancer.
将产生胞质内A颗粒簇（小鼠乳腺肿瘤病毒的前核衣壳）的DBA/2小鼠白血病细胞的丙酮固定涂片用作间接免疫荧光系统，以检测人血清中针对A颗粒的抗体。使用阳性检测血清时，在A颗粒簇部位可轻易检测到特异性荧光，表现为离散的细胞质颗粒。在43例乳腺癌患者血清中有26例（60%）发现该抗体，16例乳腺纤维腺瘤患者血清中有4例（25%）发现该抗体，而37例对照女性血清中只有4例（11%）抗体呈阳性。就乳腺癌患者而言，该抗体的出现与特定类型的肿瘤组织学无特异性关联。在相当数量的阳性病例中，该抗体在乳腺癌手术术后的不同时间段内有消失的趋势。
