Preethi Korengath Chandran, Kuttan Girija, Kuttan Ramadasan
Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur 680 555, India.
Indian J Exp Biol. 2009 Feb;47(2):113-20.
Calendula officinalis flower extract possessed significant anti-inflammatory activity against carrageenan and dextran-induced acute paw edema. Oral administration of 250 and 500 mg/kg body weight Calendula extract produced significant inhibition (50.6 and 65.9% respectively) in paw edema of animals induced by carrageenan and 41.9 and 42.4% respectively with inflammation produced by dextran. In chronic anti-inflammatory model using formalin, administration of 250 and 500 mg/kg body weight Calendula extract produced an inhibition of 32.9 and 62.3% respectively compared to controls. TNF-alpha production by macrophage culture treated with lipopolysaccharide (LPS) was found to be significantly inhibited by Calendula extract. Moreover, increased levels of proinflammatory cytokines IL- 1beta, IL-6, TNF-alpha and IFN-gamma and acute phase protein, C- reactive protein (CRP) in mice produced by LPS injection were inhibited significantly by the extract. LPS induced cyclooxygenase-2 (Cox-2) levels in mice spleen were also found to be inhibited by extract treatment. The results showed that potent anti-inflammatory response of C. officinalis extract may be mediated by the inhibition of proinflammatory cytokines and Cox-2 and subsequent prostaglandin synthesis.
金盏花花提取物对角叉菜胶和葡聚糖诱导的急性爪肿胀具有显著的抗炎活性。口服250和500mg/kg体重的金盏花提取物对由角叉菜胶诱导的动物爪肿胀产生显著抑制作用（分别为50.6%和65.9%），对葡聚糖引起的炎症分别产生41.9%和42.4%的抑制作用。在使用福尔马林的慢性抗炎模型中，与对照组相比，口服250和500mg/kg体重的金盏花提取物分别产生32.9%和62.3%的抑制作用。发现用脂多糖（LPS）处理的巨噬细胞培养物中TNF-α的产生受到金盏花提取物的显著抑制。此外，LPS注射诱导的小鼠体内促炎细胞因子IL-1β、IL-6、TNF-α和IFN-γ以及急性期蛋白C反应蛋白（CRP）水平的升高被该提取物显著抑制。提取物处理还能抑制LPS诱导的小鼠脾脏中环氧合酶-2（Cox-2）水平。结果表明，金盏花提取物强大的抗炎反应可能是通过抑制促炎细胞因子和Cox-2以及随后的前列腺素合成来介导的。
