基质辅助激光解吸电离飞行时间质谱法区分 cfiA 阴性和 cfiA 阳性脆弱拟杆菌分离株。
Differentiation of cfiA-negative and cfiA-positive Bacteroides fragilis isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
机构信息
Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium.
出版信息
J Clin Microbiol. 2011 May;49(5):1961-4. doi: 10.1128/JCM.02321-10. Epub 2011 Feb 23.
Abstract
Carbapenem resistance in Bacteroides fragilis is associated with cfiA-encoded class B metallo-beta-lactamase. cfiA-negative and cfiA-positive isolates belong to genotypically distinct groups. Of a total of 248 B. fragilis isolates included in this study, 214 were susceptible, 10 were intermediate, and 24 were resistant to meropenem. We show that matrix-assisted laser desorption ionization-time of flight mass spectrometry is able to differentiate between cfiA-negative and cfiA-positive isolates and predict carbapenem resistance in a routine laboratory setting.
摘要
脆弱拟杆菌的碳青霉烯耐药性与 cfiA 编码的 B 型金属β-内酰胺酶有关。cfiA 阴性和 cfiA 阳性分离株属于不同的基因型群体。在本研究中总共包括 248 株脆弱拟杆菌分离株，214 株为敏感株，10 株为中介株，24 株为美罗培南耐药株。我们表明基质辅助激光解吸电离飞行时间质谱能够区分 cfiA 阴性和 cfiA 阳性分离株，并在常规实验室环境中预测碳青霉烯类耐药性。
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