Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland.
J Tissue Eng. 2010 Aug 18;2010:120623. doi: 10.4061/2010/120623.
Stem cells have the capacity to differentiate into various lineages, and the ability to reliably direct stem cell fate determination would have tremendous potential for basic research and clinical therapy. Nanotopography provides a useful tool for guiding differentiation, as the features are more durable than surface chemistry and can be modified in size and shape to suit the desired application. In this paper, nanotopography is examined as a means to guide differentiation, and its application is described in the context of different subsets of stem cells, with a particular focus on skeletal (mesenchymal) stem cells. To address the mechanistic basis underlying the topographical effects on stem cells, the likely contributions of indirect (biochemical signal-mediated) and direct (force-mediated) mechanotransduction are discussed. Data from proteomic research is also outlined in relation to topography-mediated fate determination, as this approach provides insight into the global molecular changes at the level of the functional effectors.
干细胞具有分化为各种谱系的能力，而能够可靠地指导干细胞命运决定将为基础研究和临床治疗带来巨大的潜力。纳米形貌为指导分化提供了一种有用的工具，因为其特征比表面化学更持久，并且可以通过尺寸和形状进行修改，以适应所需的应用。本文研究了纳米形貌作为一种指导分化的手段，并描述了其在不同干细胞亚群中的应用，特别关注骨骼（间充质）干细胞。为了解释纳米形貌对干细胞的影响的机械基础，讨论了间接（生化信号介导）和直接（力介导）的机械转导的可能贡献。还概述了与形貌介导的命运决定相关的蛋白质组学研究数据，因为这种方法提供了对功能效应器水平上全局分子变化的深入了解。
