从中国西北部奶牛中分离出的一种新型牛病毒性腹泻病毒亚基因型的鉴定与特性分析
Identification and characterization of a novel subgenotype of bovine viral diarrhea virus isolated from dairy cattle in Northwestern China.
作者信息
Gong Xiaowei, Cao Xiaoan, Zheng Fuying, Chen Qiwei, Zhou Jizhang, Yin Hong, Liu Lihong, Cai Xuepeng
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1 Xujiaping, Chengguan, Lanzhou 730046, People's Republic of China.
出版信息
Virus Genes. 2013 Apr;46(2):375-6. doi: 10.1007/s11262-012-0861-3. Epub 2012 Dec 11.
Bovine viral diarrhea virus (BVDV) was detected by RT-PCR in 105 out of 391 samples which were collected from five dairy farms in Ningxia, China during 2010-2011. Non-cytopathogenic BVDV was isolated from 13 samples and a 230-bp fragment of the 5'-untranslated region was amplified and sequenced. While the predominant subgenotypes were BVDV-1b and BVDV-1d, a potentially novel subgenotype was identified by phylogenetic analysis, which may have implications for vaccine development.
2010年至2011年期间，在中国宁夏的五个奶牛场采集的391份样本中，有105份通过逆转录聚合酶链反应（RT-PCR）检测出牛病毒性腹泻病毒（BVDV）。从13份样本中分离出非致细胞病变性BVDV，并扩增和测序了5'-非翻译区的一个230碱基对片段。虽然主要的亚基因型是BVDV-1b和BVDV-1d，但通过系统发育分析鉴定出一种潜在的新型亚基因型，这可能对疫苗开发有影响。
Zotero 插件