Battino M, Ferri E, Gorini A, Villa R F, Rodriguez Huertas J F, Fiorella P, Genova M L, Lenaz G, Marchetti M
Istituto di Biochimica, Università di Ancona, Italy.
Membr Biochem. 1990 Jul-Sep;9(3):179-90. doi: 10.3109/09687689009025839.
The knowledge of coenzyme Q levels in tissues, organs, and subcellular compartments is of outstanding interest. A wide amount of data regarding coenzyme Q distribution and occurrence was collected in the last decades; nevertheless the data are often hard to compare because of the different extraction methods and different analytical techniques used. We have undertaken a systematic study for detecting the ubiquinone content in subcellular compartments, cells, and whole-tissue homogenates by a previously standardized HPLC method performed after an extraction procedure identical for all samples. It was confirmed that the major coenzyme Q homologue in rat tissues is coenzyme Q9; however, it was pointed out that all the rodents samples tested contain more than one coenzyme Q homologue. The coenzyme Q homologue distribution is tissue dependent with relatively high coenzyme Q10 content in brain mitochondria, irrespective of the rat strain used. There is no constant relationship of the coenzyme Q content in mitochondria and microsomes fractions. Most organisms tested (including other mammals, bird and fish specimens) have only coenzyme Q10, while the protozoan Tetrahymena pyriformis contains only coenzyme Q8.
组织、器官和亚细胞区室中辅酶Q水平的相关知识备受关注。在过去几十年里，收集了大量关于辅酶Q分布和存在情况的数据；然而，由于所使用的提取方法和分析技术不同，这些数据往往难以比较。我们采用了一种系统研究方法，通过一种先前标准化的高效液相色谱法来检测亚细胞区室、细胞和全组织匀浆中的泛醌含量，该方法在对所有样品进行相同的提取程序后进行。结果证实，大鼠组织中的主要辅酶Q同系物是辅酶Q9；然而，需要指出的是，所有测试的啮齿动物样品都含有不止一种辅酶Q同系物。辅酶Q同系物的分布因组织而异，脑线粒体中的辅酶Q10含量相对较高，与所使用的大鼠品系无关。线粒体和微粒体组分中的辅酶Q含量没有恒定的关系。大多数测试生物（包括其他哺乳动物、鸟类和鱼类标本）仅含有辅酶Q10，而原生动物梨形四膜虫仅含有辅酶Q8。
