Zhang Y, Aberg F, Appelkvist E L, Dallner G, Ernster L
Division of Medical Cell Biology, Karolinska Institutet, Novum, Huddinge, Sweden.
J Nutr. 1995 Mar;125(3):446-53. doi: 10.1093/jn/125.3.446.
Coenzyme Q is an important mitochondrial redox component and the only endogenously produced lipid-soluble antioxidant. Its tissue concentration decreases with aging and in a number of diseases; dietary supplementation of this lipid would fulfill important functions by counteracting coenzyme Q depletion. To investigate possible uptake, rats were administered 12 mumol coenzyme Q10/100 g body wt once daily by gastric intubation. The appearance of coenzyme Q10 in various tissues and blood after 6 h, 4 d or 8 d was studied. The control group of rats received rapeseed-soybean oil (the vehicle in the experimental group). Lipids were extracted with petroleum ethermethanol, and the reduced and oxidized forms of coenzyme Q9 and Q10 were separated and quantified by reversed-phase HPLC. In the plasma, the total coenzyme Q concentration was doubled after 4 d of treatment. Coenzyme Q10 was also recovered in liver homogenates, where, as in the plasma, it was largely in the reduced form. Uptake into the spleen could be to a large extent accounted for by the blood content of this organ. No dietary coenzyme Q10 was recovered in the heart or kidney. The uptake in the whole body was 2-3% of the total dose. Coenzyme Q10 found in the liver was located mainly in the lysosomes. Dietary coenzyme Q10 did not influence the endogenous biosynthesis of coenzyme Q9. This is in contrast to dietary cholesterol, which down-regulates cholesterol biosynthesis. The dietary coenzyme Q10 level in the plasma decreased to approximately 50% after 4 d. These results suggest that dietary coenzyme Q10 may play a role primarily in the blood and that no appreciable uptake occurs into tissues.
辅酶Q是一种重要的线粒体氧化还原成分，也是唯一内源性产生的脂溶性抗氧化剂。其组织浓度会随着衰老以及在多种疾病中降低；通过膳食补充这种脂质可以通过抵消辅酶Q的消耗来发挥重要作用。为了研究可能的摄取情况，通过胃管给大鼠每日一次给予12 μmol辅酶Q10/100 g体重。研究了6小时、4天或8天后辅酶Q10在各种组织和血液中的出现情况。大鼠对照组接受菜籽油 - 大豆油（实验组中的载体）。用石油醚 - 甲醇提取脂质，辅酶Q9和Q10的还原形式和氧化形式通过反相高效液相色谱法分离并定量。在血浆中，治疗4天后总辅酶Q浓度增加了一倍。辅酶Q10也在肝脏匀浆中被检测到，在那里，和在血浆中一样，它主要以还原形式存在。脾脏中的摄取在很大程度上可以由该器官的血液含量来解释。在心脏或肾脏中未检测到膳食中的辅酶Q10。全身摄取量为总剂量的2 - 3%。在肝脏中发现的辅酶Q10主要位于溶酶体中。膳食辅酶Q10不影响辅酶Q9的内源性生物合成。这与膳食胆固醇相反，膳食胆固醇会下调胆固醇生物合成。4天后血浆中的膳食辅酶Q10水平降至约50%。这些结果表明，膳食辅酶Q10可能主要在血液中发挥作用，并且在组织中没有明显的摄取。
