Takada M, Ikenoya S, Yuzuriha T, Katayama K
Biochim Biophys Acta. 1982 Feb 17;679(2):308-14. doi: 10.1016/0005-2728(82)90301-2.
Reduced and oxidized coenzyme Q10 (Q10H2 and Q10) in guinea-pig liver mitochondria were rapidly extracted and determined by high-performance liquid chromatography (HPLC). The percentages of Q10H2 as compared to the total (sum of Q10 and Q10H2) were increased by the addition of respiratory substrates such as succinate, malate and beta-hydroxybutyrate (State 4). The levels of Q10H2 in State 4 were increased more extensively with electron-transport inhibitors such as KCN, NaN3 and antimycin A. These results indicate that the method for determining Q10H2 and Q10 by HPLC is quite useful for investigation of the physiological function of coenzyme Q in mitochondria and other organelles. The reduced and oxidized coenzyme Q levels of rat liver mitochondria, which contain both coenzyme Q9 and coenzyme Q10, were measured simultaneously. The results suggest that coenzymes Q9 and Q10 play a similar role as an electron carriers. The liver microsomes of guinea-pig contained approx. 133 nmol total coenzyme Q10 per g protein. The Q10H2 levels of microsomes were increased from 46.5 to 67.5 and 64.8% with NADH and NADPH, respectively. The plasma levels of total coenzyme Q were 0.92 microgram/ml for man, 0.35 microgram/ml for guinea-pig and 0.27 microgram/ml for rat. The reduced coenzyme Q were also present in those plasma samples. The levels of reduced coenzyme Q were 51.1, 48.9 and 65.3%, respectively.
豚鼠肝脏线粒体中的还原型和氧化型辅酶Q10（Q10H2和Q10）通过高效液相色谱法（HPLC）进行快速提取和测定。添加琥珀酸、苹果酸和β-羟基丁酸等呼吸底物（状态4）后，与总量（Q10和Q10H2之和）相比，Q10H2的百分比增加。使用KCN、NaN3和抗霉素A等电子传递抑制剂时，状态4下Q10H2的水平升高更为显著。这些结果表明，通过HPLC测定Q10H2和Q10的方法对于研究辅酶Q在线粒体和其他细胞器中的生理功能非常有用。同时测量了含有辅酶Q9和辅酶Q10的大鼠肝脏线粒体中还原型和氧化型辅酶Q的水平。结果表明，辅酶Q9和Q10作为电子载体发挥类似作用。豚鼠肝脏微粒体每克蛋白质中辅酶Q10总量约为133 nmol。微粒体的Q10H2水平分别随着NADH和NADPH从46.5%增加到67.5%和64.8%。人血浆中辅酶Q总量为0.92微克/毫升，豚鼠为0.35微克/毫升，大鼠为0.27微克/毫升。这些血浆样本中也存在还原型辅酶Q。还原型辅酶Q的水平分别为51.1%、48.9%和65.3%。
