Biochemistry Group, College of Medicine, Swansea University, Swansea, UK.
Phytochemistry. 2011 Jul;72(10):975-96. doi: 10.1016/j.phytochem.2011.01.029. Epub 2011 Feb 23.
Proteins have the potential to undergo a variety of post-translational modifications and the different methods available to study these cellular processes has advanced rapidly with the continuing development of proteomic technologies. In this review we aim to detail five major post-translational modifications (phosphorylation, glycosylaion, lipid modification, ubiquitination and redox-related modifications), elaborate on the techniques that have been developed for their analysis and briefly discuss the study of these modifications in selected areas of plant science.
蛋白质有可能经历多种翻译后修饰,随着蛋白质组学技术的不断发展,研究这些细胞过程的不同方法也迅速发展。在这篇综述中,我们旨在详细介绍五种主要的翻译后修饰(磷酸化、糖基化、脂修饰、泛素化和氧化还原相关修饰),详细阐述为分析这些修饰而开发的技术,并简要讨论在植物科学的选定领域中对这些修饰的研究。