Department of Mechanical Engineering, University of Minnesota, 111 Church St. S.E., Minneapolis, MN 55455, USA.
Biosens Bioelectron. 2011 Apr 15;26(8):3482-7. doi: 10.1016/j.bios.2011.01.029. Epub 2011 Jan 28.
A low-cost, label-free, ultra-sensitive electric immunoassay is developed for the detection of swine influenza virus (SIV) H1N1. The assay is based on the excellent electrical properties of single-walled carbon nanotubes (SWCNTs). Antibody-virus complexes influence the conductance of underlying SWCNT thin film, which has been constructed by facile layer-by-layer self-assembly. The basic steps of conventional immunoassay are performed followed by the electric characterization of immunochips at the last stage. The resistance of immunochips tends to increase upon surface adsorption of macromolecules such as poly-L-lysine, anti-SIV antibodies, and SIVs during the assay. The resistance shift after the binding of SIV with anti-SIV antibody is normalized with the resistances of bare devices. The sensor selectivity tests are performed with non-SIVs, showing the normalized resistance shift of 12% as a background. The detection limit of 180 TCID(50)/ml of SIV is obtained suggesting a potential application of this assay as point-of-care detection or monitoring system. This facile CNT-based immunoassay also has the potential to be used as a sensing platform for lab-on-a-chip system.
一种低成本、无标记、超灵敏的电化学生物免疫分析方法被开发用于检测猪流感病毒（SIV）H1N1。该分析基于单壁碳纳米管（SWCNT）的优异的电学性能。抗体-病毒复合物会影响底层 SWCNT 薄膜的电导率，该薄膜是通过简单的层层自组装构建的。在最后阶段，通过对免疫芯片进行电特性分析来完成常规免疫分析的基本步骤。在分析过程中，大分子（如聚-L-赖氨酸、抗 SIV 抗体和 SIVs）的表面吸附会导致免疫芯片的电阻增加。SIV 与抗 SIV 抗体结合后的电阻变化与裸器件的电阻进行归一化。非 SIV 的传感器选择性测试显示背景下的归一化电阻变化为 12%。该方法检测 SIV 的检出限为 180TCID(50)/ml，表明该分析方法有望作为即时检测或监测系统用于临床诊断。这种基于 CNT 的电化学生物免疫分析方法也有可能作为微流控芯片系统的传感平台。
Zotero 插件
