• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

反相高效液相色谱-紫外法和薄层色谱法测定白花丹和紫花丹中白花丹素的含量

A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica.

作者信息

Unnikrishnan K P, Raja S Sudhakar, Balachandran Indira

机构信息

Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal, Malappuram-676 503, India.

出版信息

Indian J Pharm Sci. 2008 Nov;70(6):844-7. doi: 10.4103/0250-474X.49142.

DOI:10.4103/0250-474X.49142
PMID:21369461
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3040894/
Abstract

A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly higher amount of plumbagin than P. zeylanica. The HPLC and HPTLC methods described here are simple, rapid, accurate and sensitive.

摘要

已开发并验证了一种具有紫外检测的反相高效液相色谱法，用于定量测定白花丹素，即印度白花丹和锡兰白花丹根部的生物活性标志物。还开发了一种定量薄层色谱法，使用正己烷：乙酸乙酯（8:2）作为流动相。使用这两种方法测定了根部的白花丹素含量。发现印度白花丹中的白花丹素含量明显高于锡兰白花丹。这里描述的高效液相色谱法和薄层色谱法简单、快速、准确且灵敏。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f3d/3040894/20ede0a2e8cc/IJPhS-70-844-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f3d/3040894/cc418bcef245/IJPhS-70-844-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f3d/3040894/20ede0a2e8cc/IJPhS-70-844-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f3d/3040894/cc418bcef245/IJPhS-70-844-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f3d/3040894/20ede0a2e8cc/IJPhS-70-844-g002.jpg

相似文献

1
A Reverse Phase HPLC-UV and HPTLC Methods for Determination of Plumbagin in Plumbago indica and Plumbago zeylanica.反相高效液相色谱-紫外法和薄层色谱法测定白花丹和紫花丹中白花丹素的含量
Indian J Pharm Sci. 2008 Nov;70(6):844-7. doi: 10.4103/0250-474X.49142.
2
Determination of psoralen and plumbagin from its polyherbal oil formulations by an HPTLC densitometric method.采用高效薄层色谱密度法测定其多草药油制剂中的补骨脂素和白花丹醌。
J AOAC Int. 2009 May-Jun;92(3):779-84.
3
Development and Validation of HPLC Method for Quantification of Plumbagin in Plumbago Zeylanica L. Roots.用于定量测定白花丹根中白花丹醌的高效液相色谱法的建立与验证
Drug Res (Stuttg). 2023 Apr;73(4):238-242. doi: 10.1055/a-2019-4985. Epub 2023 Feb 23.
4
Comparative study of three Plumbago L. species (Plumbaginaceae) by microscopy, UPLC-UV and HPTLC.三种白花丹属（蓝雪科）植物的显微镜、UPLC-UV 和 HPTLC 比较研究。
J Nat Med. 2013 Jul;67(3):554-61. doi: 10.1007/s11418-012-0717-0. Epub 2012 Nov 15.
5
[Determination of plumbagin in different parts of Plumbago zeylanica by RP-HPLC].[采用反相高效液相色谱法测定白花丹不同部位的白花丹醌]
Zhongguo Zhong Yao Za Zhi. 2006 Oct;31(20):1684-6.
6
High-performance liquid chromatography for quantification of plumbagin, an anti-Helicobacter pylori compound of Plumbago zeylanica L.用于定量测定白花丹素（一种白花丹的抗幽门螺杆菌化合物）的高效液相色谱法
J Chromatogr A. 2005 Nov 11;1094(1-2):99-104. doi: 10.1016/j.chroma.2005.07.092. Epub 2005 Aug 15.
7
Fungal endophytes of Plumbago zeylanica L. enhances plumbagin content.白花丹的真菌内生菌可提高白花丹素含量。
Bot Stud. 2019 Sep 7;60(1):21. doi: 10.1186/s40529-019-0270-1.
8
Determination and identification of plumbagin from the roots of Plumbago zeylanica L. by liquid chromatography with tandem mass spectrometry.采用液相色谱-串联质谱法测定及鉴定白花丹根中的白花丹素。
J Chromatogr A. 2005 Aug 12;1083(1-2):141-5. doi: 10.1016/j.chroma.2005.06.030.
9
Standard operating procedure of Purification of ( Linn.) along with pharmacognostical and analytical profiles of Plumbagin.（林奈）提纯的标准操作程序以及白花丹醌的生药学和分析概况。
Ayu. 2020 Apr-Jun;41(2):117-122. doi: 10.4103/ayu.AYU_299_20. Epub 2021 Oct 23.
10
TLC and HPLC methods for the determination of plumbagin for the diagnosis of poisoning by Plumbago scandens L.用于测定白花丹素以诊断白花丹中毒的薄层色谱法和高效液相色谱法
Toxicon. 2024 Feb 23;239:107634. doi: 10.1016/j.toxicon.2024.107634. Epub 2024 Feb 1.

引用本文的文献

1
Unveiling Adulteration in Herbal Markets: MassARRAY iPLEX Assay for Accurate Identification of L.揭示草药市场中的掺假现象：用于准确鉴定L.的MassARRAY iPLEX检测法
Int J Mol Sci. 2025 Jul 24;26(15):7168. doi: 10.3390/ijms26157168.
2
Plumbagin-Loaded Glycerosome Gel as Topical Delivery System for Skin Cancer Therapy.载有白花丹醌的甘油脂质体凝胶作为皮肤癌治疗的局部给药系统。
Polymers (Basel). 2021 Mar 17;13(6):923. doi: 10.3390/polym13060923.
3
Fungal endophytes of Plumbago zeylanica L. enhances plumbagin content.白花丹的真菌内生菌可提高白花丹素含量。

本文引用的文献

1
Antioxidant properties of Plumbago zeylanica, an Indian medicinal plant and its active ingredient, plumbagin.印度药用植物白花丹及其活性成分白花丹醌的抗氧化特性。
Redox Rep. 2004;9(4):219-27. doi: 10.1179/135100004225005976.
2
A HPTLC densitometric determination of flavonoids from Passiflora alata, P. edulis, P. incarnata and P. caerulea and comparison with HPLC method.用高效薄层色谱光密度法测定翅茎西番莲、西番莲、粉红西番莲和蓝花西番莲中的黄酮类化合物，并与高效液相色谱法进行比较。
Phytochem Anal. 2004 Jul-Aug;15(4):241-8. doi: 10.1002/pca.778.
3
Antitumor and antifertility activities of plumbagin controlled release formulations.
Bot Stud. 2019 Sep 7;60(1):21. doi: 10.1186/s40529-019-0270-1.
4
Differential gene expression induced by anti-cancer agent plumbagin is mediated by androgen receptor in prostate cancer cells.抗癌剂白花丹醌诱导的差异基因表达是通过前列腺癌细胞中的雄激素受体介导的。
Sci Rep. 2018 Feb 9;8(1):2694. doi: 10.1038/s41598-018-20451-9.
5
Imbalance of the antioxidative system by plumbagin and Plumbago indica L. extract induces hepatotoxicity in mice.白花丹醌和白花丹提取物导致的抗氧化系统失衡会诱发小鼠肝毒性。
J Intercult Ethnopharmacol. 2016 Mar 24;5(2):137-45. doi: 10.5455/jice.20160301094913. eCollection 2016 Mar-Apr.
6
Śodhana: An Ayurvedic process for detoxification and modification of therapeutic activities of poisonous medicinal plants.索达纳：一种阿育吠陀排毒及改变有毒药用植物治疗活性的方法。
Anc Sci Life. 2015 Apr-Jun;34(4):188-97. doi: 10.4103/0257-7941.160862.
7
Drosera peltata Smith var. lunata (Buch.-Ham.) C. B. Clarke as a feasible source of plumbagin: phytochemical analysis and antifungal activity assay.圆叶茅膏菜（Smith var. lunata (Buch.-Ham.) C. B. Clarke）作为一种可行的补骨脂素来源：植物化学分析和抗真菌活性测定。
World J Microbiol Biotechnol. 2014 Feb;30(2):737-45. doi: 10.1007/s11274-013-1495-x.
白花丹醌控释制剂的抗肿瘤和抗生育活性。
Indian J Exp Biol. 1997 Apr;35(4):374-9.