Krzyzanowska Justyna, Janda Bogdan, Pecio Lukasz, Stochmal Anna, Oleszek Wieslaw, Czubacka Anna, Przybys Marcin, Doroszewska Teresa
Institute of Soil Science and Plant Cultivation-State Research Institute, Department of Biochemistry, ul. Czartoryskich 8, 24-100 Pulawy, Poland.
J AOAC Int. 2011 Jan-Feb;94(1):43-50.
Nine polyphenols in the aerial parts of Mentha longifolia have been separated by chromatographic techniques. Their structures have been confirmed by HPLC/electrospray ionization-MS/MS. The compounds identified included rosmarinic acid, salvianolic acid L, dedihydro-salvianolic acid, luteolin-glucuronide, luteolin-diglucuronide, luteolin-glucopyranosyl-rhamnopyranoside, and eriodictyol-glucopyranosyl-rhamnopyranoside. The extracts of M. longifolia and M. piperita field plants, in vitro plants, callus tissues, and cell suspension cultures were profiled, and their polyphenol composition was compared in different tissues and quantified using ultra-performance column liquid chromatography (UPLC)/triple-quadrupole-MS in the selected-ion recording detection mode. Determination of desired compounds was based on calibration curves obtained for standards, which were previously isolated from M. longifolia aerial parts. The UPLC profiles revealed considerable differences in the synthesis of secondary metabolites among samples coming from field plants, in vitro plants, callus tissues, and cell suspension cultures. Plant tissues coming from field cultivation (for both M. piperita and M. longifolia) contained several phenolic compounds (flavonoids and phenolic acids), whereas plants from in vitro conditions, callus tissues, and suspension cultures contained only a few of them. Rosmarinic acid dominated in all of these samples. These results show that under in vitro conditions, the metabolism of phenolics undergoes a fundamental change.
通过色谱技术已分离出长叶薄荷地上部分的九种多酚。其结构已通过高效液相色谱/电喷雾电离-串联质谱法得到确认。鉴定出的化合物包括迷迭香酸、丹酚酸L、去氢丹酚酸、木犀草素-葡萄糖醛酸苷、木犀草素-二葡萄糖醛酸苷、木犀草素-葡萄糖吡喃糖基-鼠李糖吡喃糖苷和圣草酚-葡萄糖吡喃糖基-鼠李糖吡喃糖苷。对长叶薄荷和胡椒薄荷的田间植株、离体植株、愈伤组织和细胞悬浮培养物的提取物进行了分析，并比较了它们在不同组织中的多酚组成，并采用超高效液相色谱（UPLC）/三重四极杆质谱在选择离子记录检测模式下进行定量。目标化合物的测定基于从长叶薄荷地上部分预先分离得到的标准品所获得的校准曲线。UPLC分析结果显示，来自田间植株、离体植株、愈伤组织和细胞悬浮培养物的样品在次生代谢产物合成方面存在显著差异。来自田间种植的植株（胡椒薄荷和长叶薄荷）含有几种酚类化合物（黄酮类和酚酸类），而来自离体条件、愈伤组织和悬浮培养物的植株仅含有其中的少数几种。迷迭香酸在所有这些样品中占主导地位。这些结果表明，在离体条件下，酚类物质的代谢发生了根本性变化。
