Sajewicz Mieczysław, Staszek Dorota, Wojtal Łukasz, Kowalska Teresa, Hajnos Michał Ł, Waksmundzka-Hajnos Monika
University of Silesia, Institute of Chemistry, 9 Szkolna St, Katowice, Poland.
J AOAC Int. 2011 Jan-Feb;94(1):71-6.
This study is focused on an important family of the sage (Salvia) species, with Salvia officinalis L. having a long-established position in European traditional medicine. Binary fingerprints (chromatographic profiles) of six different sage species were compared using HPLC coupled with two different detectors: the diode-array detector and the evaporative light-scattering detector. Advantages of using binary fingerprinting over single-detector fingerprinting are demonstrated and discussed, with selected examples. Experimental data are provided for a comparison of the chemical composition of sage samples originating from two harvesting seasons (2007 and 2008). A number of phytochemical standards (i.e., certain phenolic acids, flavonoids, and coumarin) were used that allowed identification and semiquantitative estimation of these particular compounds in the analyzed methanol extracts.
本研究聚焦于鼠尾草属的一个重要植物家族，其中鼠尾草在欧洲传统医学中有着长期确立的地位。使用高效液相色谱（HPLC）结合两种不同的检测器：二极管阵列检测器和蒸发光散射检测器，比较了六种不同鼠尾草物种的二元指纹图谱（色谱图）。通过实例展示并讨论了使用二元指纹图谱相对于单检测器指纹图谱的优势。提供了实验数据，用于比较来自两个收获季节（2007年和2008年）的鼠尾草样品的化学成分。使用了多种植物化学标准品（即某些酚酸、黄酮类化合物和香豆素），以便对分析的甲醇提取物中的这些特定化合物进行鉴定和半定量估计。
