Laboratory of Nutrition and Metabolism, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes 3900, SP, Ribeirao Preto, Brazil.
Eur J Appl Physiol. 2011 Nov;111(11):2663-70. doi: 10.1007/s00421-011-1891-6. Epub 2011 Mar 11.
The aim of this study was to evaluate the effect of creatine supplementation on homocysteine (Hcy) metabolism after acute aerobic and anaerobic exercise. A total of 112 Wistar rats were divided into four groups: aerobic exercise (A), aerobic exercise plus creatine supplementation (ACr), anaerobic exercise (An), and anaerobic exercise plus creatine-supplemented (AnCr). Creatine supplementation consisted of the addition of 2% creatine monohydrate to the diet. After 28 days, the rats performed an acute moderate aerobic exercise bout (1 h swimming with 4% of total body weight load) or an acute intense anaerobic exercise bout (6 × 30-s vertical jumps into the water with a 30-s rest between jumps, with 50% of total body weight load). The animals were killed before (pre) and at 0, 2, and 6 h (n = 8) after acute exercise. Plasma Hcy concentration increased significantly (P < 0.05) up to 2 h after anaerobic exercise (An group: pre 8.7 ± 1.2, 0 h 13.2 ± 2.3, 2 h 13.5 ± 4.2, and 6 h 12.1 ± 2.2, μmol/l). The same did not occur in acute aerobic exercised animals. Nevertheless, creatine supplementation significant decreased (P < 0.05) homocysteine concentration independent of exercise intensity (AnCr group: pre 17%, 0 h 80%, 2 h 107%, and 6 h 48%; ACr group: pre 17%, 0 h 19%, 2 h 28%, and 6 h 27%). Increased S-adenosylhomocysteine was also found in the An group. In conclusion, acute intense anaerobic exercise increased plasma Hcy concentration. On the other hand, creatine supplementation decreased plasma Hcy independent of exercise intensity.
本研究旨在评估补充肌酸对急性有氧和无氧运动后同型半胱氨酸（Hcy）代谢的影响。将 112 只 Wistar 大鼠分为四组：有氧运动（A）、有氧运动加肌酸补充（ACr）、无氧运动（An）和无氧运动加肌酸补充（AnCr）。肌酸补充由饮食中添加 2%肌酸一水合物组成。28 天后，大鼠进行急性中度有氧运动（1 小时游泳，负重 4%体重）或急性剧烈无氧运动（6×30 秒垂直跳入水中，每跳之间休息 30 秒，负重 50%体重）。动物在急性运动前（pre）和 0、2 和 6 小时（n=8）时被处死。无氧运动后，血浆 Hcy 浓度显著升高（P<0.05），直至 2 小时（An 组：pre 8.7±1.2、0 h 13.2±2.3、2 h 13.5±4.2 和 6 h 12.1±2.2 μmol/L）。而急性有氧运动的动物则没有出现这种情况。然而，无论运动强度如何，肌酸补充都显著降低了同型半胱氨酸浓度（AnCr 组：pre 17%、0 h 80%、2 h 107%和 6 h 48%；ACr 组：pre 17%、0 h 19%、2 h 28%和 6 h 27%）。An 组还发现 S-腺苷同型半胱氨酸增加。总之，急性剧烈无氧运动增加了血浆 Hcy 浓度。另一方面，肌酸补充降低了血浆 Hcy 浓度，与运动强度无关。
