Deminice Rafael, Rosa Flávia Troncon, Franco Gabriel Silveira, da Cunha Selma Freirede Carvalho, de Freitas Ellen Cristini, Jordao Alceu Afonso
Laboratory of Nutrition and Metabolism, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes 3900, Ribeirão Prêto, Sao Paulo, Brazil,
Eur J Nutr. 2014 Sep;53(6):1355-61. doi: 10.1007/s00394-013-0636-1. Epub 2013 Dec 8.
The purpose of the study is to evaluate the effects of creatine supplementation on homocysteine (Hcy) plasma levels after acute exercise in humans.
Twenty-three young (under-20) soccer players were divided into 2 groups: creatine (Cr)- and placebo (Pla)-supplemented groups. The supplementation was performed in double-blind controlled manner using creatine or placebo tablets with 0.3 g/kg during 7 days. Before and after 7 days of supplementation, the athletes performed an acute high-intensity sprint exercise (two consecutive running-based anaerobic sprint test protocol consisted in 6 × 35 m sprint with 10 s between them). Blood samples were collected before and after 7 days of supplementation as well as 0 and 1 h after exercise protocol.
Homocysteine concentration significant increased (P < 0.05) 1 h after acute exercise (18%). Acute exercise also decreased red blood cell S-adenosylmethionine (SAM) 30% with no changes in SAM/SAH ratio. Seven days of creatine supplementation were able to increase (P < 0.05) plasma creatine concentration (Pla 130.1 ± 21.7 vs Cr 1,557.2 ± 220.3 μmol/L) as well as decrease (P < 0.05) plasma guanidinoacetic acid (33%). Controversially, creatine supplementation did not change Hcy plasma level after 7-day supplementation (Pla 6.9 ± 0.2 vs Cr 7.2 ± 0.2 μmol/L) or after acute exercise (Pla 8.2 ± 0.3 vs Cr 8.4 ± 0.3 μmol/L). No changes in plasma vitamin B12 and folate as well as cysteine and methionine were found.
Seven days of creatine supplementation does not avoid increased plasma Hcy induced by acute sprint exercise in humans.
本研究旨在评估补充肌酸对人体急性运动后血浆同型半胱氨酸（Hcy）水平的影响。
23名年轻（20岁以下）足球运动员被分为两组：补充肌酸（Cr）组和补充安慰剂（Pla）组。采用双盲对照方式，在7天内使用肌酸或安慰剂片，剂量为0.3 g/kg。在补充7天前后，运动员进行了一次急性高强度冲刺运动（连续两次基于跑步的无氧冲刺测试方案，包括6次35米冲刺，每次冲刺间隔10秒）。在补充7天前后以及运动方案前后0小时和1小时采集血样。
急性运动后1小时，同型半胱氨酸浓度显著升高（P < 0.05）（升高18%）。急性运动还使红细胞S-腺苷甲硫氨酸（SAM）降低30%，而SAM/SAH比值无变化。补充7天肌酸能够使血浆肌酸浓度升高（P < 0.05）（安慰剂组为130.1±21.7，肌酸组为1557.2±220.3μmol/L），同时使血浆胍基乙酸降低（P < 0.05）（降低33%）。有争议的是，补充7天肌酸后，血浆Hcy水平在补充后（安慰剂组为6.9±0.2，肌酸组为7.2±0.2μmol/L）或急性运动后（安慰剂组为8.2±0.3，肌酸组为8.4±0.3μmol/L）均未改变。血浆维生素B12、叶酸以及半胱氨酸和蛋氨酸均未发现变化。
补充7天肌酸并不能避免人体急性冲刺运动诱导的血浆Hcy升高。
