Department of Nutrition, China Medical University, Taichung, Taiwan, Republic of China.
J Agric Food Chem. 2011 Apr 27;59(8):4306-14. doi: 10.1021/jf200117m. Epub 2011 Mar 18.
Adlay ( Coix lachryma-jobi L. var. ma-yuen Stapf) is a grass crop and is reported to protect against various diseases such as cancer. To investigate the effect of the ethanolic extract of adlay bran (ABE) on drug-metabolizing enzymes and glutathione-related antioxidant enzymes in rats, three groups of eight male Sprague-Dawley rats each were fed a control diet or a diet containing 5 or 10% ABE for 4 weeks. Significant decreases in microsomal cytochrome P-450 (CYP) 1A1-catalyzed ethoxyresorufin O-deethylation, CYP2C-catalyzed diclofenac 4-hydroxylation, CYP2D-catalyzed dextromethorphan O-demethylation, and CYP3A-catalyzed testosterone 6β-hydroxylation in the liver and CYP1A1-catalyzed ethoxyresorufin O-deethylation in the lungs of rats fed ABE were observed. Immunoblot analyses also showed decreases of CYP1A1, 1A2, 2C6, 2C11, 2D1, 2E1, 3A1, and 3A2 in the liver and CYP1A1 in the lungs. Furthermore, rats fed the 10% ABE diet had a higher glutathione content and glutathione peroxidase, glutathione reductase, and glutathione S-transferase activities in the lungs, but such an increase was not noted in the liver. Inhibition of various CYP-catalyzed enzyme reactions by ABE in rat and human liver microsomes had also been shown. The results of this study indicate that ABE feeding may suppress CYP enzyme activities and CYP protein expression in the liver and lungs of rats. Moreover, the increase of the antioxidant potential by ABE is tissue-specific.
薏苡仁（Coix lachryma-jobi L. var. ma-yuen Stapf）是一种草本作物，据报道具有预防各种疾病的作用，如癌症。为了研究薏苡仁麸皮乙醇提取物（ABE）对大鼠药物代谢酶和谷胱甘肽相关抗氧化酶的影响，将 3 组 8 只雄性 Sprague-Dawley 大鼠分别喂食对照饲料或含 5%或 10% ABE 的饲料 4 周。结果发现，ABE 喂养组大鼠肝脏中细胞色素 P-450（CYP）1A1 催化的乙氧基Resorufin O-去乙基化、CYP2C 催化的双氯芬酸 4-羟化、CYP2D 催化的右美沙芬 O-去甲基化和 CYP3A 催化的睾酮 6β-羟化作用以及肺中 CYP1A1 催化的乙氧基Resorufin O-去乙基化作用显著降低。免疫印迹分析还显示，ABE 喂养组大鼠肝脏中 CYP1A1、1A2、2C6、2C11、2D1、2E1、3A1 和 3A2 以及肺中 CYP1A1 的表达减少。此外，喂食 10% ABE 饲料的大鼠肺组织中的谷胱甘肽含量和谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽 S-转移酶活性更高，但在肝脏中没有观察到这种增加。ABE 还抑制了大鼠和人肝微粒体中各种 CYP 催化的酶反应。本研究结果表明，ABE 喂养可能会抑制大鼠肝和肺中 CYP 酶活性和 CYP 蛋白表达。此外，ABE 增加抗氧化能力具有组织特异性。
