猪颞下颌关节盘的局部细胞密度分布和耗氧量：一项培养物研究。
Regional cell density distribution and oxygen consumption rates in porcine TMJ discs: an explant study.
机构信息
Department of Bioengineering, Clemson University, Clemson, SC, USA.
出版信息
Osteoarthritis Cartilage. 2011 Jul;19(7):911-8. doi: 10.1016/j.joca.2011.03.002. Epub 2011 Mar 10.
OBJECTIVE
To determine the regional cell density distribution and basal oxygen consumption rates (based on tissue volume and cell number) of temporomandibular joint (TMJ) discs and further examine the impact of oxygen tension on these rates.
DESIGN
TMJ discs from pigs aged 6-8 months were divided into five regions: anterior, intermediate, posterior, lateral and medial. The cell density was determined using confocal laser scanning microscopy. The change in oxygen tension was recorded while TMJ disc explants were cultured in sealed metabolism chambers. The volume based oxygen consumption rate of explants was determined by theoretical curve-fitting of the recorded oxygen tension data with the Michaelis-Menten equation. The rate on a per-cell basis was calculated based on the cell density measurements and volume based rate measured in another group of discs.
RESULTS
The overall cell density [mean, 95% confidence interval (CI)] was 51.3 (21.3-81.3) × 10(6) cells/mL wet tissue. Along the anteroposterior axis, the anterior band had 25.5% higher cell density than the intermediate zone (P<0.02) and 29.1% higher than the posterior band (P<0.008). Along the mediolateral axes, the medial region had 26.2% higher cell density than the intermediate zone (P<0.04) and 25.4% higher than the lateral region (P<0.045). The overall volume and cell based maximum oxygen consumption rates were 1.44 (0.44-2.44) μmol/mL wet tissue/h and 28.7 (12.2-45.2)nmol/10(6)cells/h, respectively. The central regions (intermediate, lateral, and medial) had significantly higher volume based (P<0.02) and cell based (P<0.005) oxygen consumption rates than the anterior and posterior bands. At high oxygen tension, the oxygen consumption rate remained constant, but dropped as oxygen tension fell below 5%.
CONCLUSIONS
The TMJ disc had higher cell density and oxygen consumption rates than articular cartilage reported in the literature. These results suggest that a steeper oxygen gradient may exist in the TMJ disc and may be vulnerable to pathological events that impede nutrient supply.
目的
确定颞下颌关节（TMJ）盘的区域细胞密度分布和基础耗氧率（基于组织体积和细胞数量），并进一步研究氧张力对这些速率的影响。
设计
将 6-8 月龄猪的 TMJ 盘分为五个区域：前、中、后、外和内。使用共聚焦激光扫描显微镜确定细胞密度。在密封代谢室中培养 TMJ 盘标本时，记录氧张力的变化。通过将记录的氧张力数据与米氏方程的理论曲线拟合，确定标本的基于体积的耗氧率。根据细胞密度测量值和另一组盘的基于体积的速率测量值，计算基于细胞的速率。
结果
总细胞密度[平均值，95%置信区间（CI）]为 51.3（21.3-81.3）×10（6）个细胞/mL 湿组织。在前-后轴上，前带的细胞密度比中间带高 25.5%（P<0.02），比后带高 29.1%（P<0.008）。在中-外侧轴上，内侧区域的细胞密度比中间带高 26.2%（P<0.04），比外侧区域高 25.4%（P<0.045）。总容积和细胞基础最大耗氧率分别为 1.44（0.44-2.44）μmol/mL 湿组织/h 和 28.7（12.2-45.2）nmol/10（6）个细胞/h。中央区域（中间、外侧和内侧）的基于体积（P<0.02）和基于细胞（P<0.005）的耗氧率明显高于前带和后带。在高氧张力下，耗氧率保持不变，但当氧张力降至 5%以下时，耗氧率下降。
结论
TMJ 盘的细胞密度和耗氧率高于文献报道的关节软骨。这些结果表明，TMJ 盘可能存在更陡的氧梯度，并且可能容易受到阻碍营养供应的病理事件的影响。
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