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鉴定水族馆鱼类中的新型隐孢子虫。

Identification of novel Cryptosporidium species in aquarium fish.

机构信息

Department of Fisheries, Faculty of Marine Natural Resources, Khoramshahr Marine Science and Technology, Khoramshahr, Khouzestan, Iran.

出版信息

Vet Parasitol. 2010 Nov 24;174(1-2):43-8. doi: 10.1016/j.vetpar.2010.08.006. Epub 2010 Aug 17.

DOI:10.1016/j.vetpar.2010.08.006
PMID:20832174
Abstract

Little is known about the prevalence and genotypes of Cryptosporidium in fish. The present study investigated the prevalence of Cryptosporidium species in 200 aquarium fish of 39 different species in Western Australia by PCR amplification at the 18S rRNA locus. A total of 21 positives were detected by PCR (10.5% prevalence) from 13 different species of fish. Nineteen of these isolates were successfully sequenced. Of these, 12 were similar or identical to previously described species/genotypes of Cryptosporidium, while the remaining seven isolates appeared to represent three novel species.

摘要

关于鱼类中隐孢子虫的流行情况和基因型知之甚少。本研究通过在 18S rRNA 基因座进行 PCR 扩增,调查了西澳大利亚州 200 种 39 种不同鱼类中隐孢子虫的流行情况。共从 13 种鱼类中检测到 21 个 PCR 阳性(10.5%的患病率)。其中 19 个分离株成功测序。这些分离株中,12 个与先前描述的隐孢子虫种/基因型相似或相同,而其余 7 个分离株似乎代表了三个新种。

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