Animal Pathology Department, Faculty of Veterinary, University of Santiago de Compostela, 27002-Lugo, Spain.
Vet Parasitol. 2011 Jun 30;179(1-3):277-82. doi: 10.1016/j.vetpar.2011.02.014. Epub 2011 Feb 25.
The analysis of the capability of the nematode trapping-fungus Duddingtonia flagrans to adapt to the cyathostomin egg-output in horses was evaluated. Fecal samples from 196 pasturing autochthonous Pura Raza Galega horses were collected from the rectum and then divided according to the egg-output into three groups: ≤ 300, 310-800 and >800 eggs per gram feces. Four doses of chlamydospores (0.1, 0.2, 0.4 and 0.8 × 10(6)/100g feces) were directly spread onto fecal pats on the ground, remaining one without treatment as control. Fecal pats confirmed the presence of gastrointestinal nematode larvae belonging to strongylid cyathostomins (Cyathostomum and Gyalocephalus spp). An overall 94% (95% CI 91, 97) percentage of reduction was obtained, and an increase in the activity of the trapping-fungi simultaneously to the rising in the number of cyathostomin eggs and larvae in the coprocultures was detected. A significantly highest reduction of the cyathostomin L3 in the coprocultures with more than 800 EPG was found, which indicates that Df trapping activity is larvae nematode density-dependant. The present research showed the high biological activity of D. flagrans against nematode larvae can adjust to the cyathostomin egg-output, and underlines its efficacy as a practical method for the control of these parasites in grazing horses.
评价了捕食线虫真菌多形轮枝菌（Duddingtonia flagrans）适应马属动物寄生性胃肠道线虫卵排放量的能力。从直肠采集了 196 匹放牧的纯种 Galega 马的粪便样本，然后根据卵排放量将其分为三组：≤300、310-800 和>800 个卵/克粪便。将四个剂量的厚垣孢子（0.1、0.2、0.4 和 0.8×10（6）/100g 粪便）直接撒在地上的粪便垫上，留一个不做处理作为对照。粪便垫证实存在属于强捻线虫（Cyathostomum 和 Gyalocephalus spp.）的胃肠道线虫幼虫。总体减少了 94%（95%CI 91，97），同时在共培养物中寄生性胃肠道线虫卵和幼虫数量增加时，捕食真菌的活性也增加了。在共培养物中，超过 800 EPG 的寄生性胃肠道线虫幼虫的减少量显著更高，这表明 Df 捕食活性与线虫幼虫密度有关。本研究表明，D. flagrans 对线虫幼虫具有很高的生物学活性，能够适应寄生性胃肠道线虫的卵排放量，强调了其作为控制放牧马寄生虫的实用方法的功效。
