Abd Ellah Mahmoud Rushdi
Clinical Laboratory Diagnosis, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt.
Vet Med Int. 2011 Feb 14;2011:613602. doi: 10.4061/2011/613602.
The goal of the current paper is to explore the role of liver biopsy as a tool in detection of hepatic oxidative stress, with brief notes on different types of free radicals, antioxidants, hepatic and blood oxidative stress, and lipid peroxidation. Hepatic oxidative stress was investigated for many years in human and animals, but most of the studies performed in animals were concerned with studying oxidative status in the liver tissues after slaughtering or euthanasia. However, in human medicine, a large number of studies were implemented to investigate the status of antioxidants in liver biopsy specimens. Similar studies are required in animals, as the changes in hepatic antioxidants and formation of lipid peroxide give a good idea about the condition of the liver. On the other hand, hepatic disease may present without significant effect on blood oxidative status, and, consequently, the best way to detect the status of hepatic oxidants and antioxidants is through measuring in liver biopsy. Measuring antioxidants status directly in the liver tissues gives an accurate estimation about the condition of the liver, permits the diagnosis of hepatic dysfunction, and helps to determine the degree of deterioration in the hepatic cells.
本文的目的是探讨肝活检作为检测肝脏氧化应激工具的作用，并简要介绍不同类型的自由基、抗氧化剂、肝脏和血液氧化应激以及脂质过氧化。多年来，人们一直在人和动物身上研究肝脏氧化应激，但大多数在动物身上进行的研究都关注屠宰或安乐死后肝脏组织中的氧化状态。然而，在人类医学中，已经开展了大量研究来调查肝活检标本中的抗氧化剂状态。动物也需要进行类似的研究，因为肝脏抗氧化剂的变化和脂质过氧化物的形成能很好地反映肝脏的状况。另一方面，肝脏疾病可能出现而对血液氧化状态没有显著影响，因此，检测肝脏氧化剂和抗氧化剂状态的最佳方法是通过肝活检进行测量。直接在肝脏组织中测量抗氧化剂状态可以准确评估肝脏状况，有助于诊断肝功能障碍，并有助于确定肝细胞的恶化程度。
