Discipline of Anatomy and Pathology, School of Medical Sciences, University of Adelaide, Adelaide, SA, Australia.
J Clin Periodontol. 2011 Jun;38(6):532-41. doi: 10.1111/j.1600-051X.2011.01714.x. Epub 2011 Mar 21.
Previous studies have shown a higher incidence of alveolar bone loss in patients with rheumatoid arthritis (RA) and that patients with periodontitis are at a greater risk of developing RA. The aim of this study was to develop an animal model to assess the relationship between pre-existing periodontitis and experimental arthritis (EA).
Periodontitis was first induced in mice by oral gavage with Porphyromonas gingivalis followed by EA using the collagen antibody-induced arthritis model. These animals were compared with animals with periodontitis alone, EA alone and no disease (controls). Visual changes in paw swelling were assessed to determine clinical development of EA. Alveolar bone and joint changes were assessed using micro-CT, histological analyses and immunohistochemistry. Serum levels of C-reactive protein were used to monitor systemic inflammation.
Mice with pre-existing periodontitis developed more severe arthritis, which developed at a faster rate. Mice with periodontitis only also showed evidence of loss of bone within the radiocarpal joint. There was also evidence of alveolar bone loss in mice with EA alone.
The results of this study indicate that pre-existing periodontitis exacerbated experimental arthritis in a mouse model.
先前的研究表明，类风湿关节炎（RA）患者的牙槽骨丧失发生率更高，且牙周炎患者患 RA 的风险更大。本研究旨在建立一种动物模型，以评估先前存在的牙周炎与实验性关节炎（EA）之间的关系。
首先通过口腔灌胃用牙龈卟啉单胞菌诱导小鼠牙周炎，然后使用胶原抗体诱导的关节炎模型诱导 EA。将这些动物与仅患有牙周炎、仅患有 EA 以及无疾病的动物（对照组）进行比较。通过观察爪子肿胀的视觉变化来评估 EA 的临床发展。使用 micro-CT、组织学分析和免疫组织化学来评估牙槽骨和关节的变化。血清 C 反应蛋白水平用于监测全身炎症。
患有预先存在的牙周炎的小鼠发生了更严重的关节炎，且发展速度更快。仅患有牙周炎的小鼠的桡腕关节内也出现了骨丢失的证据。仅患有 EA 的小鼠也有牙槽骨丧失的证据。
本研究结果表明，预先存在的牙周炎在小鼠模型中加重了实验性关节炎。
