使用单酶扩增片段长度多态性对猪源多杀性巴氏杆菌多杀亚种分离株进行分型
Use of single-enzyme amplified fragment length polymorphism for typing Pasteurella multocida subsp. multocida isolates from pigs.
作者信息
Moreno A M, Baccaro M R, Ferreira A J P, Pestana De Castro A F
机构信息
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Sao Paulo, Brazil.
出版信息
J Clin Microbiol. 2003 Apr;41(4):1743-6. doi: 10.1128/JCM.41.4.1743-1746.2003.
Abstract
Single-enzyme amplified fragment length polymorphism (SE-AFLP) analyses were used to differentiate 97 isolates of porcine Pasteurella multocida subsp. multocida. The strains, isolated from animals with pneumonia, rhinitis, and septicemia, were classified as capsular types A, D, and F. SE-AFLP showed a discriminatory index of 0.87 and identified 18 different profiles.
摘要
采用单酶切扩增长度多态性分析（SE-AFLP）对97株多杀性巴氏杆菌多杀亚种进行鉴别。这些菌株从患有肺炎、鼻炎和败血症的动物中分离得到，分为A、D和F荚膜型。SE-AFLP显示鉴别指数为0.87，并鉴定出18种不同的图谱。
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