Center for Pharmaceutical Biotechnology, University of Illinois, 900 S. Ashland Ave., Chicago, IL 60607, USA.
Antimicrob Agents Chemother. 2011 Jun;55(6):2989-91. doi: 10.1128/AAC.00183-11. Epub 2011 Mar 28.
The indigenous methyltransferase RlmN modifies A2503 in 23S rRNA. A recently described rlmN mutation in a clinical Staphylococcus aureus isolate decreases susceptibility to linezolid and was thought to increase the extent of A2503 modification. However, we show that the mutation in fact abolishes RlmN activity, resulting in a lack of A2503 modification. Since many mutations could inactivate the rlmN gene, our findings unveil a potential mechanism for future linezolid resistance in clinical strains.
本土甲基转移酶 RlmN 修饰 23S rRNA 中的 A2503。最近在临床分离的金黄色葡萄球菌中描述了一个 rlmN 突变，该突变降低了对利奈唑胺的敏感性，并被认为增加了 A2503 修饰的程度。然而，我们表明该突变实际上使 RlmN 失活，导致缺乏 A2503 修饰。由于许多突变可能使 rlmN 基因失活，因此我们的发现揭示了临床菌株中未来对利奈唑胺产生耐药性的潜在机制。
