Slaughter L, Carson D A, Jensen F C, Holbrook T L, Vaughan J H
J Exp Med. 1978 Nov 1;148(5):1429-34. doi: 10.1084/jem.148.5.1429.
Peripheral blood mononuclear cells from 10 patients with rheumatoid arthritis and 9 control subjects were cultured in vitro for 30 days with and without infection by Epstein-Barr virus. All cultures showed polyclonal stimulation of B cells as indicated by rising levels of IgM in the culture supernates, reaching maximal at 18-24 days, and with no quantitative or kinetic difference between the RA and control cells. IgM anti-IgG was also produced in both groups and maximally at 18-24 days, but in greater quantity by the RA lymphocytes. The anti-IgG made by the RA lymphocytes was more easily absorbed by solid phase IgG than was the anti-IgG made by the normal lymphocytes and thus was judged to be of higher affinity. RA lymphocytes uninfected with EBV had higher transformation scores than did the normal controls and developed spontaneously into permanent cell lines in six instances.
从10例类风湿性关节炎患者和9例对照受试者获取外周血单个核细胞，在有或无爱泼斯坦-巴尔病毒感染的情况下进行30天的体外培养。所有培养物均显示B细胞受到多克隆刺激，表现为培养上清液中IgM水平升高，在18 - 24天达到最高，类风湿性关节炎患者和对照细胞之间在数量或动力学上无差异。两组均产生IgM抗IgG，同样在18 - 24天达到最高，但类风湿性关节炎淋巴细胞产生的量更多。类风湿性关节炎淋巴细胞产生的抗IgG比正常淋巴细胞产生的抗IgG更容易被固相IgG吸收，因此判断其亲和力更高。未感染EBV的类风湿性关节炎淋巴细胞比正常对照具有更高的转化分数，并且在6例中自发发展为永久细胞系。
