Charité-Universitätsmedizin, Center of Experimental Applied Cutaneous Physiology, Department of Dermatology and Allergology, Berlin, Germany.
J Biomed Opt. 2011 Mar;16(3):035002. doi: 10.1117/1.3555183.
The human organism has developed a protection system against the destructive effect of free radicals. The aim of the present study was to investigate the extent of exogenous stress factors such as disinfectant and IR-A radiation on the skin, and their influence on the kinetics of carotenoids distribution during the recovery process. Ten healthy volunteers were assessed with resonance spectroscopy using an Argon-laser at 488 nm to excite the carotenoids in vivo. Additionally, Raman-confocal-micro-spectroscopy measurements were performed using a model 3510 Skin Composition Analyzer with spatially resolved measurements down to 30 μm. The measurements were performed at a baseline of 20, 40, 60, and 120 min after an external stressor consisting either of water-filtered infrared A (wIRA) with 150 mW∕cm(2) or 1 ml∕cm(2) of an alcoholic disinfectant. Both Raman methods were capable to detect the infrared-induced depletion of carotenoids. Only Raman-microspectroscopy could reveal the carotenoids decrease after topical disinfectant application. The carotenoid-depletion started at the surface. After 60 min, recovery starts at the surface while deeper parts were still depleted. The disinfectant- and wIRA-induced carotenoid depletion in the epidermis recovers from outside to inside and probably delivered by sweat and sebaceous glands. We could show that the Raman microscopic spectroscopy is suited to analyze the carotenoid kinetic of stress effects and recovery.
人体已经发展出一种保护系统来对抗自由基的破坏作用。本研究的目的是调查消毒剂和 IR-A 辐射等外源应激因素对皮肤的影响程度，以及它们对类胡萝卜素在恢复过程中分布动力学的影响。使用 Argon 激光在 488nm 激发体内类胡萝卜素，对 10 名健康志愿者进行共振光谱评估。此外，还使用型号为 3510 的皮肤成分分析仪进行拉曼共聚焦显微镜测量，可进行空间分辨率测量，深度低至 30μm。测量在基线 20、40、60 和 120 分钟后进行，外部应激源为水过滤红外 A（wIRA），强度为 150mW∕cm(2)或 1ml∕cm(2)的酒精消毒剂。两种拉曼方法都能够检测到红外诱导的类胡萝卜素耗竭。只有拉曼显微镜能够揭示局部应用消毒剂后类胡萝卜素的减少。类胡萝卜素的耗竭始于表面。60 分钟后，表面开始恢复，而更深的部位仍在耗竭。表皮中由消毒剂和 wIRA 引起的类胡萝卜素耗竭从外向内恢复，可能通过汗液和皮脂腺输送。我们表明，拉曼显微镜适合分析应激效应和恢复过程中的类胡萝卜素动力学。
