James Rachel, Kim Yongsoo, Hockberger Philip E, Szele Francis G
Department of Physiology, Anatomy and Genetics, University of Oxford Oxford, UK.
Front Neurosci. 2011 Mar 21;5:30. doi: 10.3389/fnins.2011.00030. eCollection 2011.
Neuroblasts born in the adult subventricular zone (SVZ) migrate long distances in the rostral migratory stream (RMS) to the olfactory bulbs where they integrate into circuitry as functional interneurons. As very little was known about the dynamic parameters of SVZ neuroblast migration, we used two-photon time-lapse microscopy to analyze migration in acute slices. This involved analyzing 3D stacks of images over time and uncovered several novel aspects of SVZ migration: chains remain stable, cells can be immotile for extensive periods, morphology does not necessarily correlate with motility, neuroblasts exhibit local exploratory motility, dorsoventral migration occurs throughout the striatal SVZ, and neuroblasts turn at distinctive angles. We investigated these novel findings in the SVZ and RMS from the population to the single cell level. In this review we also discuss some technical considerations when setting up a two-photon microscope imaging system. Throughout the review we identify several unsolved questions about SVZ neuroblast migration that might be addressed with current or emerging techniques.
成体脑室下区（SVZ）产生的神经母细胞在吻侧迁移流（RMS）中远距离迁移至嗅球，在那里它们作为功能性中间神经元整合到神经回路中。由于对SVZ神经母细胞迁移的动态参数了解甚少，我们使用双光子延时显微镜来分析急性脑片中的迁移情况。这包括随时间分析三维图像堆栈，并揭示了SVZ迁移的几个新方面：细胞链保持稳定，细胞可长时间静止不动，形态不一定与运动性相关，神经母细胞表现出局部探索性运动，在整个纹状体SVZ中发生背腹迁移，且神经母细胞以独特角度转弯。我们在从群体到单细胞水平的SVZ和RMS中研究了这些新发现。在这篇综述中，我们还讨论了设置双光子显微镜成像系统时的一些技术考量。在整个综述中，我们确定了几个关于SVZ神经母细胞迁移的未解决问题，这些问题可能可以用当前或新兴技术来解决。
