采用彗星试验检测过氧化氢对人肝癌细胞的遗传毒性。
Genotoxic effect induced by hydrogen peroxide in human hepatoma cells using comet assay.
机构信息
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Al-Fateh University for Medical Sciences, Tripoli, Libya.
出版信息
Libyan J Med. 2010 Jan 13;5. doi: 10.3402/ljm.v5i0.4637.
BACKGROUND
Hydrogen peroxide (H(2)O(2)) is a common reactive oxygen intermediate generated by various forms of oxidative stress.
AIM
The aim of this study was to investigate the DNA damage capacity of H(2)O(2) in HepG2 cells.
METHODS
Cells were treated with H(2)O(2) at concentrations of 25 µM or 50 µM for 5 min, 30 min, 40 min, 1 h, or 24 h in parallel. The extent of DNA damage was assessed by the comet assay.
RESULTS
Compared to the control, DNA damage by 25 and 50 µM H(2)O(2) increased significantly with increasing incubation time up to 1 h, but it was not increased at 24 h.
CONCLUSIONS
Our findings confirm that H(2)O(2) is a typical DNA damage-inducing agent and thus is a good model system to study the effects of oxidative stress. DNA damage in HepG2 cells increased significantly with H(2)O(2) concentration and time of incubation but later decreased likely due to DNA repair mechanisms and antioxidant enzymes.
背景
过氧化氢（H₂O₂）是各种形式氧化应激产生的常见活性氧中间体。
目的
本研究旨在研究 H₂O₂在 HepG2 细胞中的 DNA 损伤能力。
方法
细胞分别用 25µM 或 50µM 的 H₂O₂处理 5 分钟、30 分钟、40 分钟、1 小时或 24 小时。通过彗星试验评估 DNA 损伤程度。
结果
与对照组相比，25µM 和 50µM 的 H₂O₂在孵育 1 小时内显著增加 DNA 损伤，但在 24 小时后没有增加。
结论
我们的研究结果证实，H₂O₂是一种典型的 DNA 损伤诱导剂，因此是研究氧化应激影响的良好模型系统。HepG2 细胞中的 DNA 损伤随着 H₂O₂浓度和孵育时间的增加而显著增加，但后来由于 DNA 修复机制和抗氧化酶的作用而减少。
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