血培养中大肠埃希菌、肺炎克雷伯菌和铜绿假单胞菌的鉴定：一种三色彩肽核酸荧光原位杂交检测方法的多中心性能评估。
Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in blood cultures: a multicenter performance evaluation of a three-color peptide nucleic acid fluorescence in situ hybridization assay.
机构信息
Columbia University Medical Center, NewYork-Presbyterian Hospital, New York, New York, USA.
出版信息
J Clin Microbiol. 2011 Jun;49(6):2259-61. doi: 10.1128/JCM.02250-10. Epub 2011 Apr 13.
Abstract
A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence in situ hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186/186) of the Escherichia coli isolates, 99.1% (109/110) of the Klebsiella pneumoniae isolates, and 95.8% (46/48) of the Pseudomonas aeruginosa isolates in this study. Negative assay results were correctly obtained for 162 of 165 other GNB (specificity, 98.2%).
摘要
一项多中心评估研究评估了一种新的三色彩肽核酸荧光原位杂交检测方法，该方法可直接鉴定革兰氏阴性杆菌（GNB）血培养阳性的分离株。在这项研究中，该检测方法正确鉴定了 100%（186/186）的大肠杆菌分离株、99.1%（109/110）的肺炎克雷伯菌分离株和 95.8%（46/48）的铜绿假单胞菌分离株。对于 165 株其他 GNB（特异性，98.2%）中的 162 株，该检测方法获得了正确的阴性检测结果。
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