Columbia University Medical Center, NewYork-Presbyterian Hospital, New York, New York, USA.
J Clin Microbiol. 2011 Jun;49(6):2259-61. doi: 10.1128/JCM.02250-10. Epub 2011 Apr 13.
A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence in situ hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186/186) of the Escherichia coli isolates, 99.1% (109/110) of the Klebsiella pneumoniae isolates, and 95.8% (46/48) of the Pseudomonas aeruginosa isolates in this study. Negative assay results were correctly obtained for 162 of 165 other GNB (specificity, 98.2%).
一项多中心评估研究评估了一种新的三色彩肽核酸荧光原位杂交检测方法,该方法可直接鉴定革兰氏阴性杆菌(GNB)血培养阳性的分离株。在这项研究中,该检测方法正确鉴定了 100%(186/186)的大肠杆菌分离株、99.1%(109/110)的肺炎克雷伯菌分离株和 95.8%(46/48)的铜绿假单胞菌分离株。对于 165 株其他 GNB(特异性,98.2%)中的 162 株,该检测方法获得了正确的阴性检测结果。