BD GeneOhm MRSA 去色酶检测法在实时 PCR 检测鼻腔标本中耐甲氧西林金黄色葡萄球菌中的性能。
Performance of the BD GeneOhm MRSA achromopeptidase assay for real-time PCR detection of methicillin-resistant Staphylococcus aureus in nasal specimens.
机构信息
NorthShore University HealthSystem, Evanston, Illinois 60201, USA.
出版信息
J Clin Microbiol. 2011 Jun;49(6):2266-8. doi: 10.1128/JCM.02431-10. Epub 2011 Apr 20.
Abstract
We evaluated the BD GeneOhm MRSA achromopeptidase (ACP) assay, which incorporates a new specimen preparation approach. A total of 1,216 leftover nasal samples were tested; using culture as the gold standard, the sensitivity and specificity were 92% and 94.6%, respectively. The new lysis method provides good sensitivity and simplifies specimen preparation.
摘要
我们评估了 BD GeneOhm MRSA 无色素酶（ACP）检测法，该方法采用了一种新的标本制备方法。共检测了 1216 份剩余的鼻拭子样本；以培养为金标准，该检测法的灵敏度和特异性分别为 92%和 94.6%。新的裂解方法提供了良好的灵敏度并简化了标本制备。
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