Tierney J T, Larkin E P
Appl Environ Microbiol. 1978 Sep;36(3):432-7. doi: 10.1128/aem.36.3.432-437.1978.
A review of virus thermal inactivation data published in the literature demonstrated variations in reported virus resistance. Examination of the methods used indicated that numerous studies were made by heat processing virus suspensions in test tubes. Duplication of some of the methods using milk suspensions of poliovirus 1 showed virus persistence after heating as a result of uneven temperature distribution inside the test tubes. Unless the containers (preferably sealed ampoules or capillary tubes) are completely submerged in the water bath and agitated vigorously, apparent virus persistence may be encountered.
对文献中发表的病毒热灭活数据的回顾表明，所报道的病毒抗性存在差异。对所使用方法的检查表明，许多研究是通过在试管中对病毒悬液进行热处理来进行的。使用脊髓灰质炎病毒1的牛奶悬液重复其中一些方法，结果表明由于试管内温度分布不均，加热后病毒仍会持续存在。除非容器（最好是密封安瓿或毛细管）完全浸没在水浴中并剧烈搅拌，否则可能会出现明显的病毒持续存在现象。
