反相高效液相色谱法测定毛冬青中毛冬青甲素的含量

[Determination of tortoside A in Ilicis pubescentis by RP-HPLC].

作者信息

Li Shuli, Zhou Yuan, Tu Pengfei

机构信息

Binzhou Vocational College, Binzhou 256603, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Nov;36(22):3146-8.

PMID:22375396

Abstract

OBJECTIVE

To establish a RP-HPLC method for the determination of tortoside A in Ilex pubences.

METHOD

Kromasil-C18 (4.6 mm x 250 mm, 5 microm) column was used in HPLC with mobile phase acetonitrite-0.1% H3PO4 (17:83), the column temperature was 30 degrees C, the flow rate was 1 mL x min(-1), the detection wavelength was set at 210 nm, and inject volume was 10 microL RESULT: Tortoside A was well separated under the established conditions, the liner range of tortoside A was 26.05-521.00 microg (r = 0.999 9, n = 6), and the average recovery was 98.42%.

CONCLUSION

It was the first time to establish the RP-HPLC method with accuracy, good reproducibility for determining the content of tortoside A in I. Pubescentis.

摘要

目的

建立测定毛冬青中毛冬青皂苷甲的反相高效液相色谱法。

方法

采用Kromasil-C18（4.6 mm×250 mm，5μm）色谱柱，以乙腈-0.1%磷酸（17:83）为流动相，柱温30℃，流速1 mL·min⁻¹，检测波长210 nm，进样量10μL。结果：在该条件下毛冬青皂苷甲得到良好分离，毛冬青皂苷甲的线性范围为26.05 - 521.00μg（r = 0.999 9，n = 6），平均回收率为98.42%。