Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
Macromol Rapid Commun. 2011 Jun 16;32(12):905-11. doi: 10.1002/marc.201100125. Epub 2011 Apr 26.
A robust synthetic strategy where polysaccharide derivative precursors react through aqueous Diels-Alder chemistry without the involvement of catalysts and coupling reagents, allowing for the direct encapsulation of positive and negative proteins within biodegradable hydrogels. The results demonstrated that the aqueous Diels-Alder chemistry provides an extremely selective reaction and proceeds with high efficiency for polysaccharide bioconjugation. This synthetic approach uniquely allows for the direct fabrication of biologically functionalized gels with ideal structures, which provides a competitive alternative to conventional conjugation techniques such as click chemistry.
一种稳健的合成策略，其中多糖衍生物前体通过水相 Diels-Alder 化学反应，无需使用催化剂和偶联试剂，可直接将正负蛋白包封在可生物降解的水凝胶中。结果表明，水相 Diels-Alder 化学提供了一种极其选择性的反应，多糖偶联反应高效进行。这种合成方法独特地允许直接制备具有理想结构的生物功能化凝胶，为传统的偶联技术（如点击化学）提供了一种有竞争力的替代方案。
