Department ofDiagnostic Radiology, Kitasato University School of Medicine, Kitasato, Sagamihara, Kanagawa, Japan.
Mol Imaging. 2011 Oct;10(5):377-85. doi: 10.2310/7290.2010.00057. Epub 2011 Apr 26.
Gaussia luciferase (Gluc) is a secreted reporter, and its expression in living animals can be assessed by in vivo bioluminescence imaging (BLI) or blood assays. We characterized Gluc as an in vivo reporter in comparison with firefly luciferase (Fluc). Mice were inoculated subcutaneously with tumor cells expressing both Fluc and Gluc and underwent Fluc BLI, Gluc BLI, blood assays of Gluc activity, and caliper measurement. In Gluc BLI, the signal from the tumor peaked immediately and then decreased rapidly. In the longitudinal monitoring, all measures indicated an increase in tumor burden early after cell inoculation. However, the increase reached plateaus in Gluc BLI and Fluc BLI despite a continuous increase in the caliper measurement and Gluc blood assay. Significant correlations were found between the measures, and the correlation between the blood signal and caliper volume was especially high. Gluc allows tumor monitoring in mice and should be applicable to dual-reporter assessment in combination with Fluc. The Gluc blood assay appears to provide a reliable indicator of viable tumor burden, and the combination of a blood assay and in vivo BLI using Gluc should be promising for quantifying and localizing the tumors.
海肾荧光素酶（Gluc）是一种分泌型报告基因，其在活体动物中的表达可通过活体生物发光成像（BLI）或血液检测进行评估。我们将 Gluc 与萤火虫荧光素酶（Fluc）进行了比较，以确定其是否可作为活体报告基因。将同时表达 Fluc 和 Gluc 的肿瘤细胞皮下接种于小鼠，然后进行 Fluc BLI、Gluc BLI、Gluc 活性的血液检测和卡尺测量。在 Gluc BLI 中，肿瘤的信号立即达到峰值，然后迅速下降。在纵向监测中，所有指标均表明细胞接种后早期肿瘤负担增加。然而，尽管卡尺测量和 Gluc 血液检测持续增加，Gluc BLI 和 Fluc BLI 中的增加均达到平台期。这些指标之间存在显著相关性，且血液信号与卡尺体积之间的相关性尤其高。Gluc 可用于监测小鼠肿瘤，并且应该可以与 Fluc 联合用于双报告基因评估。Gluc 血液检测似乎可提供肿瘤活度的可靠指标，使用 Gluc 的血液检测与活体 BLI 的结合有望用于定量和定位肿瘤。
