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转基因小鼠的自主生物发光发射。

Autonomous bioluminescence emission from transgenic mice.

作者信息

Kiszka Kamila A, Dullin Christian, Steffens Heinz, Koenen Tanja, Rothermel Ellen, Alves Frauke, Gregor Carola

机构信息

Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.

University Medical Center Göttingen, Institute for Diagnostic and Interventional Radiology, Göttingen, Germany.

出版信息

Sci Adv. 2025 Jul 11;11(28):eads0463. doi: 10.1126/sciadv.ads0463. Epub 2025 Jul 9.

DOI:10.1126/sciadv.ads0463
PMID:40632853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12239958/
Abstract

The bacterial bioluminescence system can be applied to produce autonomous bioluminescence in mammalian cells. Until now, the system has only been stably inserted into cells cultured in vitro. Here, we report the generation of an autobioluminescent transgenic mouse line constitutively expressing the genes of the bacterial bioluminescence system, enabling substrate-free in vivo luminescence imaging of a living mammal.

摘要

细菌生物发光系统可用于在哺乳动物细胞中产生自主生物发光。到目前为止，该系统仅被稳定地插入体外培养的细胞中。在此，我们报告了一种组成型表达细菌生物发光系统基因的自发光转基因小鼠品系的产生，从而能够对活体哺乳动物进行无底物的体内发光成像。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ed/12239958/00cca9429aaa/sciadv.ads0463-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ed/12239958/6d9576763363/sciadv.ads0463-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ed/12239958/50d7ce8643de/sciadv.ads0463-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ed/12239958/498db04166b7/sciadv.ads0463-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ed/12239958/00cca9429aaa/sciadv.ads0463-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ed/12239958/6d9576763363/sciadv.ads0463-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ed/12239958/50d7ce8643de/sciadv.ads0463-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ed/12239958/498db04166b7/sciadv.ads0463-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/23ed/12239958/00cca9429aaa/sciadv.ads0463-f4.jpg

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本文引用的文献

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Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system.利用细菌生物发光系统对单个哺乳动物细胞进行自主生物发光成像。
Proc Natl Acad Sci U S A. 2019 Dec 26;116(52):26491-26496. doi: 10.1073/pnas.1913616116. Epub 2019 Dec 2.
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Restoration of brain circulation and cellular functions hours post-mortem.恢复死后数小时的大脑循环和细胞功能。
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Identification of Factors Complicating Bioluminescence Imaging.
生物发光成像复杂因素的识别
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Single-cell bioluminescence imaging of deep tissue in freely moving animals.在自由活动的动物中对深部组织进行单细胞生物发光成像。
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A Transgenic Dual-Luciferase Reporter Mouse for Longitudinal and Functional Monitoring of T Cells .用于 T 细胞纵向和功能监测的转基因双荧光素酶报告鼠
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Transgenic mouse model for imaging of interleukin-1β-related inflammation in vivo.用于体内白细胞介素-1β相关炎症成像的转基因小鼠模型。
Sci Rep. 2015 Nov 24;5:17205. doi: 10.1038/srep17205.
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Expression of a humanized viral 2A-mediated lux operon efficiently generates autonomous bioluminescence in human cells.人源化病毒2A介导的lux操纵子的表达在人细胞中有效地产生自主生物发光。
PLoS One. 2014 May 2;9(5):e96347. doi: 10.1371/journal.pone.0096347. eCollection 2014.
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Gaussia luciferase for bioluminescence tumor monitoring in comparison with firefly luciferase.用于生物发光肿瘤监测的海肾荧光素酶与萤火虫荧光素酶的比较。
Mol Imaging. 2011 Oct;10(5):377-85. doi: 10.2310/7290.2010.00057. Epub 2011 Apr 26.
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Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell line.细菌荧光素酶基因盒（lux）在哺乳动物细胞系中的自主生物发光表达。
PLoS One. 2010 Aug 27;5(8):e12441. doi: 10.1371/journal.pone.0012441.
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Uptake kinetics and biodistribution of 14C-D-luciferin--a radiolabeled substrate for the firefly luciferase catalyzed bioluminescence reaction: impact on bioluminescence based reporter gene imaging.14C-D-荧光素的摄取动力学和生物分布——一种用于萤火虫荧光素酶催化生物发光反应的放射性标记底物：对基于生物发光的报告基因成像的影响
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