Lotfi-Kamran Mohammad Hossein, Jafari Abbas Ali, Falah-Tafti Abbas, Tavakoli Ehsan, Falahzadeh Mohammad Hossein
Assistant Professor, Department of Prosthodontics, School of Dentistry, Yazd University of Medical Sciences, Yazd, Iran.
Dent Res J (Isfahan). 2009 Spring;6(1):23-7.
Oral candidiasis is a common opportunistic infection in diabetic patients. Presence of denture in the oral cavity of diabetic patients can promote Candida colonization and results in the higher incidence of oral and systemic candidiasis. The general purpose of the present study was to evaluate and compare Candida colonization in denture of diabetic patients and non-diabetic control group.
In current case-control study, samples for mycological examinations were collected from the palatal impression surface of maxillary dentures from 92 edentulous patients including 46 dia-betic and 46 non-diabetic denture wearers. All samples were cultured directly on sabouraud agar me-dium and isolated colonies were counted and identified based on specific tests. Data were statistically analyzed using Mann-Whitney and Spearman correlation tests.
The higher density of isolated colonies was seen in diabetic group in compare with control group (P = 0.0001). There was a statistically significant correlation between the blood glucose level (P = 0.0001) and the duration of denture usage (P = 0.022) with the colonization of Candida on denture of diabetic patients. C. albicans was the most common isolated Candida species in both groups, though diabetic patients with dentures had more non-albicans Candida isolated from their dentures compared to non-diabetic patients.
Mycological findings from the present study revealed that diabetes mellitus can in-crease colonization of Candida in denture and mouth. By elimination of local and systemic factors in diabetic patients and improving their oral health care, Candida colonization and the risk of oral and systemic candidiasis will be decreased.
口腔念珠菌病是糖尿病患者常见的机会性感染。糖尿病患者口腔中佩戴假牙可促进念珠菌定植，导致口腔和全身念珠菌病的发病率更高。本研究的总体目的是评估和比较糖尿病患者与非糖尿病对照组假牙上念珠菌的定植情况。
在当前的病例对照研究中，从92名无牙患者的上颌假牙腭面印模表面采集用于真菌学检查的样本，其中包括46名佩戴假牙的糖尿病患者和46名非糖尿病患者。所有样本直接接种于沙保弱琼脂培养基上，对分离出的菌落进行计数，并通过特定试验进行鉴定。数据采用曼-惠特尼检验和斯皮尔曼相关性检验进行统计学分析。
与对照组相比，糖尿病组分离菌落的密度更高（P = 0.0001）。糖尿病患者假牙上念珠菌定植与血糖水平（P = 0.0001）和假牙使用时间（P = 0.022）之间存在统计学显著相关性。白色念珠菌是两组中最常见的分离念珠菌种类，不过佩戴假牙的糖尿病患者假牙上分离出的非白色念珠菌比非糖尿病患者更多。
本研究的真菌学结果表明，糖尿病可增加念珠菌在假牙和口腔中的定植。通过消除糖尿病患者的局部和全身因素并改善其口腔卫生保健，念珠菌定植以及口腔和全身念珠菌病的风险将降低。
