十种伊朗产牙膏的体外抗菌活性。

An in vitro antimicrobial activity of ten Iranian-made toothpastes.

作者信息

Sadeghi Mostafa, Assar Shokrollah

机构信息

Associate Professor, Department of Restorative Dentistry, School of Dentistry, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

出版信息

Dent Res J (Isfahan). 2009 Fall;6(2):87-92.

PMID:21528037

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3075461/

Abstract

BACKGROUND

Antimicrobial agents have been used as a chemotherapeutic agent to improve oral health. This in vitro study was carried out to determine the antimicrobial activity of ten Iranian-made toothpastes against commonly found bacteria in the oral cavity.

METHODS

The microorganisms used in this study were Streptococcus mutans, Streptococcus sanguis, Actinomyces viscosus and Candida albicans. Sterile discs impregnated with 10 Iranian-made toothpastes; Paveh, Saviz, Latifeh II, Bath, Darugar II, Darugar I, Close up, Tage, Pooneh III and Nasim, which were separately used on agar plates. Crest Cavity Protection toothpaste and Sterile pyrogenfree distilled water were used as positive and negative controls, respectively. The samples were tested in triplicate, at full strength, 1:1 and 1:3 dilutions. Inhibition zones were measured in millimeter after 48 hr. The data were analyzed by the ANOVA and t-test.

RESULTS

All tested toothpastes demonstrated an antimicrobial activity. The antimicrobial activity of Bath on S. mutans, Paveh on S. sanguis, Paveh, Saviz, Latifeh III and Darugar II on C. albicans were similar to the activity of Crest Cavity Protection. The antimicrobial activity of Pooneh III and Nasim on S. mutans, Bath on S. sanguis and A. viscosus, and Bath and Pooneh III on C. albicans were significantly higher and the others were significantly lower than the positive control. While, the activity of Crest Cavity Protection was the same as Pooneh III, it showed a weaker activity compared with Bath.

CONCLUSION

Apart from Bath and Pooneh III, the other Iranian-made toothpastes tested in this study showed a lower antimicrobial activity compared to Crest Cavity Protection.

摘要

背景

抗菌剂已被用作一种化学治疗剂来改善口腔健康。本体外研究旨在确定十种伊朗产牙膏对口腔中常见细菌的抗菌活性。

方法

本研究中使用的微生物为变形链球菌、血链球菌、粘性放线菌和白色念珠菌。用10种伊朗产牙膏浸渍无菌圆盘；帕韦赫、萨维兹、拉蒂费二世、巴斯、达鲁加尔二世、达鲁加尔一世、佳洁士、塔杰、普内赫三世和纳西姆，分别用于琼脂平板上。佳洁士防蛀牙膏和无菌无热原蒸馏水分别用作阳性和阴性对照。样品以原液、1:1和1:3稀释度一式三份进行测试。48小时后测量抑菌圈大小（以毫米为单位）。数据采用方差分析和t检验进行分析。

结果

所有测试牙膏均表现出抗菌活性。巴斯对变形链球菌、帕韦赫对血链球菌、帕韦赫、萨维兹、拉蒂费三世和达鲁加尔二世对白色念珠菌的抗菌活性与佳洁士防蛀牙膏相似。普内赫三世和纳西姆对变形链球菌、巴斯对血链球菌和粘性放线菌以及巴斯和普内赫三世对白色念珠菌的抗菌活性显著高于阳性对照，其他的则显著低于阳性对照。虽然佳洁士防蛀牙膏的活性与普内赫三世相同，但与巴斯相比，其活性较弱。