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人类疱疹病毒6型主要衣壳蛋白基因的鉴定、克隆及表达

Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.

作者信息

Littler E, Lawrence G, Liu M Y, Barrell B G, Arrand J R

机构信息

Department of Molecular Biology, Christie Hospital & Holt Radium Institute, Manchester, United Kingdom.

出版信息

J Virol. 1990 Feb;64(2):714-22. doi: 10.1128/JVI.64.2.714-722.1990.

DOI:10.1128/JVI.64.2.714-722.1990
PMID:2153237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249165/
Abstract

DNA sequence analysis of part of the human herpesvirus 6 (HHV-6) genome led to the identification of an open reading frame with amino acid sequence homology to the major capsid proteins (MCP) of other HHVs. DIAGON analysis showed that the closest homology was with human cytomegalovirus. Plasmids were constructed which were shown to express the HHV-6 MCP as either the entire open reading frame or as portions of it, and the recombinant-produced proteins were used to raise antisera. The antisera were shown by immunofluorescence to react with HHV-6-infected lymphoblastoid cells and in Western blots with a 135-kilodalton protein specific to HHV-6-infected cells. The recombinant protein expressed from the entire HHV-6 MCP gene was detected only weakly in Western blot assays with normal HHV-6-positive human sera as a probe.

摘要

对人类疱疹病毒6型（HHV - 6）基因组部分区域进行的DNA序列分析，导致鉴定出一个开放阅读框，其氨基酸序列与其他疱疹病毒的主要衣壳蛋白（MCP）具有同源性。DIAGON分析表明，其与人类巨细胞病毒的同源性最为接近。构建了质粒，这些质粒被证明可表达整个开放阅读框或其部分的HHV - 6 MCP，并且重组产生的蛋白质被用于制备抗血清。通过免疫荧光显示，抗血清可与HHV - 6感染的淋巴母细胞发生反应，在蛋白质免疫印迹中可与HHV - 6感染细胞特有的一种135千道尔顿的蛋白质发生反应。以正常HHV - 6阳性人血清作为探针，在蛋白质免疫印迹分析中，从整个HHV - 6 MCP基因表达的重组蛋白仅被微弱检测到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdb/249165/b645c65978f8/jvirol00057-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdb/249165/f1b3f198f66c/jvirol00057-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdb/249165/00c5a716da36/jvirol00057-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdb/249165/b645c65978f8/jvirol00057-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdb/249165/f1b3f198f66c/jvirol00057-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdb/249165/00c5a716da36/jvirol00057-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bdb/249165/b645c65978f8/jvirol00057-0267-a.jpg

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