Staden R
Nucleic Acids Res. 1986 Jan 10;14(1):217-31. doi: 10.1093/nar/14.1.217.
I describe the current status of our sequence analysis software. The package contains a comprehensive suite of programs for managing large shotgun sequencing projects, a program containing 61 functions for analysing single sequences and a program for comparing pairs of sequences for similarity. The programs that have been described before have been improved by the addition of new functions and by being made very much easier to use. The major interactive programs have 125 pages of online help available from within them. Several new programs are described including screen editing of aligned gel readings for shotgun sequencing projects; a method to highlight errors in aligned gel readings, new methods for searching for putative signals in sequences. We use the programs on a VAX computer but the whole package has been rewritten to make it easy to transport it to other machines. I believe the programs will now run on any machine with a FORTRAN77 compiler and sufficient memory. We are currently putting the programs onto an IBM PC XT/AT and another micro running under UNIX.
我描述了我们序列分析软件的当前状态。该软件包包含一套用于管理大型鸟枪法测序项目的综合程序、一个包含61个用于分析单序列功能的程序以及一个用于比较成对序列相似性的程序。之前描述过的程序通过添加新功能和使其更易于使用得到了改进。主要的交互式程序中有125页的在线帮助。描述了几个新程序,包括用于鸟枪法测序项目的比对凝胶读数的屏幕编辑;一种突出显示比对凝胶读数中错误的方法、在序列中搜索假定信号的新方法。我们在VAX计算机上使用这些程序,但整个软件包已被重写以便于移植到其他机器上。我相信这些程序现在可以在任何具有FORTRAN77编译器和足够内存的机器上运行。我们目前正在将这些程序安装到IBM PC XT/AT和另一台运行UNIX的微型计算机上。