脂质过氧化启动的机制。反对铁（II）-铁（III）复合物需求的证据。
The mechanism of initiation of lipid peroxidation. Evidence against a requirement for an iron(II)-iron(III) complex.
作者信息
Aruoma O I, Halliwell B, Laughton M J, Quinlan G J, Gutteridge J M
机构信息
Department of Biochemistry, University of London King's College, Strand, U.K.
出版信息
Biochem J. 1989 Mar 1;258(2):617-20. doi: 10.1042/bj2580617.
Abstract
When Fe2+ ions are added to rat-liver microsomes, lipid peroxidation begins after a short lag period. Fe2+-dependent peroxidation in the first few minutes of the incubation can be increased by adding Fe3+, ascorbic acid or Pb2+ ions; these stimulations are not additive. By contrast, Pb2+ ions inhibit peroxidation of microsomes in the presence of Fe3+/ascorbate or Fe3+-ADP/NADPH. In liposomes made from ox-brain phospholipids, Fe2+-dependent peroxidation is stimulated slightly by Fe3+, but much more so by ascorbic acid, Al3+ or Pb2+; these stimulations are not additive. Liposomal peroxidation in the presence of Fe3+/ascorbate is inhibited by Pb2+ or Al3+. These results argue against the participation of an Fe2+-Fe3+-O2 complex, or a critical 1:1 ratio of Fe2+ to Fe3+, in the initiation of lipid peroxidation in liposomes and rat-liver microsomes.
摘要
当向大鼠肝脏微粒体中添加Fe2+离子时，脂质过氧化在短暂的延迟期后开始。孵育最初几分钟内依赖Fe2+的过氧化反应可通过添加Fe3+、抗坏血酸或Pb2+离子而增强；这些刺激作用并非累加的。相比之下，在存在Fe3+/抗坏血酸盐或Fe3+-ADP/NADPH的情况下，Pb2+离子会抑制微粒体的过氧化反应。在用牛脑磷脂制成的脂质体中，Fe3+对依赖Fe2+的过氧化反应有轻微刺激作用，但抗坏血酸、Al3+或Pb2+的刺激作用更强；这些刺激作用并非累加的。在存在Fe3+/抗坏血酸盐的情况下，脂质体的过氧化反应会被Pb2+或Al3+抑制。这些结果表明，Fe2+-Fe3+-O2复合物或Fe2+与Fe3+的关键1:1比例并不参与脂质体和大鼠肝脏微粒体中脂质过氧化的起始过程。
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本文引用的文献
1
The requirement for ferric in the initiation of lipid peroxidation by chelated ferrous iron.螯合亚铁引发脂质过氧化过程中对三价铁的需求。
Biochem Biophys Res Commun. 1983 Mar 29;111(3):777-84. doi: 10.1016/0006-291x(83)91366-9.
2
Effect of hydrogen peroxide on the initiation of microsomal lipid peroxidation.过氧化氢对微粒体脂质过氧化起始的影响。
Biochem Pharmacol. 1983 Jan 1;32(1):123-7. doi: 10.1016/0006-2952(83)90663-9.
3
Ferrous ion-EDTA-stimulated phospholipid peroxidation. A reaction changing from alkoxyl-radical- to hydroxyl-radical-dependent initiation.亚铁离子-乙二胺四乙酸刺激的磷脂过氧化。一种从依赖烷氧基自由基引发转变为依赖羟基自由基引发的反应。
Biochem J. 1984 Dec 15;224(3):697-701. doi: 10.1042/bj2240697.
4
Radiation-induced generation and properties of lipid hydroperoxide in liposomes.
Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Dec;44(6):615-25. doi: 10.1080/09553008314551681.
5
The role of superoxide and hydroxyl radicals in phospholipid peroxidation catalysed by iron salts.超氧化物和羟基自由基在铁盐催化的磷脂过氧化反应中的作用。
FEBS Lett. 1982 Dec 27;150(2):454-8. doi: 10.1016/0014-5793(82)80788-6.
6
Superoxide dependent lipid peroxidation.超氧化物依赖性脂质过氧化作用。
Fed Proc. 1981 Feb;40(2):179-82.
7
A micromethod for the determination of ferroxidase (ceruloplasmin) in human serums.一种测定人血清中铁氧化酶（铜蓝蛋白）的微量方法。
Clin Chem. 1967 Feb;13(2):142-50.
8
The importance of free radicals and catalytic metal ions in human diseases.自由基和催化金属离子在人类疾病中的重要性。
Mol Aspects Med. 1985;8(2):89-193. doi: 10.1016/0098-2997(85)90001-9.
9
Lipid peroxidation and cellular damage in toxic liver injury.中毒性肝损伤中的脂质过氧化与细胞损伤
Lab Invest. 1985 Dec;53(6):599-623.
10
Delayed, ferrous iron-dependent peroxidation of rat liver microsomes.大鼠肝微粒体的延迟性、亚铁离子依赖性过氧化作用。
Arch Biochem Biophys. 1987 Dec;259(2):372-81. doi: 10.1016/0003-9861(87)90503-0.
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