College of Life Science and Technology, Inner Mongolia Normal University, Hohhot, 010022, Inner Mongolia, China.
Key Laboratory of Biodiversity Conservation and Sustainable Utilization for College and University of Inner Mongolia Autonomous Region, Hohhot, 010022, China.
Arch Microbiol. 2021 Dec 28;204(1):92. doi: 10.1007/s00203-021-02724-6.
A novel Gram-negative strain, designated X100-76, was isolated from Daihai Lake in Inner Mongolia, Republic of China. The strain was non-motile, non-spore-forming, long-rod-shaped, oxidase positive and catalase positive. Colonies incubated at 33 °C on 2216 marine agar medium for 3 days were circular, smooth, transparent, convex with clear edges, orange-red in colour and approximately 1.0 mm in diameter. Growth occurred at pH 6.5-12 (optimum pH 7.5), in 0-5.0% (w/v) NaCl (optimum 1.5%, w/v) and at 4-40 °C (optimum 28-33 °C). Phylogenetic analysis based on 16S rRNA gene sequences showed that X100-76 belongs to the genus Mongoliitalea and is most closely related to Mongoliitalea lutea MIM18 with 98.3% sequence similarity. The total genome size of X100-76 was 4,816,617 bp with a G + C content of 39.6%. The digital DNA-DNA hybridization and average nucleotide identity values between strain X100-76 and M. lutea MIM18 were both below the recommended cut-off values. Chemotaxonomic analysis revealed iso-C, Sum Feature 4 (anteiso-C B and/or iso-C I), Cω6c, Sum Feature 9 (C 10-methyl and/or iso-Cω9c), C and iso-C G as the major fatty acids, menaquinone MK-7 as the major isoprenoid quinone, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol as the major cellular polar lipids. The results of polyphasic analysis demonstrated that X100-76 represents a novel species within the genus Mongoliitalea, for which the name Mongoliitalea daihaiensis sp. nov. is proposed. The type strain is X100-76 (= CGMCC 1.18762 = KCTC 82458).
一株新的革兰氏阴性菌株，命名为 X100-76，从中国内蒙古自治区的岱海湖中分离得到。该菌株为非运动、非孢子形成的长杆状，氧化酶阳性，过氧化氢酶阳性。在 2216 号海洋琼脂培养基上于 33°C 孵育 3 天的菌落呈圆形、光滑、透明、凸起、边缘清晰，呈橙红色，直径约 1.0mm。该菌株在 pH 值为 6.5-12（最适 pH 值为 7.5）、0-5.0%（w/v）NaCl（最适 1.5%，w/v）和 4-40°C（最适 28-33°C）的条件下均可生长。基于 16S rRNA 基因序列的系统发育分析表明，X100-76 属于 Mongoliitalea 属，与最接近的 Mongoliitalea lutea MIM18 的序列相似性为 98.3%。X100-76 的全基因组大小为 4816617bp，GC 含量为 39.6%。菌株 X100-76 与 M. lutea MIM18 之间的全基因组 DNA-DNA 杂交值和平均核苷酸同一性值均低于推荐的截止值。化学分类分析表明，菌株 X100-76 的主要脂肪酸为 iso-C、Sum Feature 4（anteiso-C B 和/或 iso-C I）、Cω6c、Sum Feature 9（C 10-甲基和/或 iso-Cω9c）、C 和 iso-C G；主要类异戊二烯醌为menaquinone MK-7；主要的细胞极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱和磷脂酰肌醇。多相分析的结果表明，X100-76 代表了 Mongoliitalea 属中的一个新种，因此提议将其命名为岱海 Mongoliitalea daihaiensis sp. nov.。模式菌株为 X100-76（=CGMCC 1.18762=KCTC 82458）。
