China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
College of Life Science and Technology, Xinjiang University, Urumchi 830046, PR China.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):665-670. doi: 10.1099/ijs.0.030122-0. Epub 2011 May 6.
A Gram-negative-staining, rod-shaped, non-motile and pink bacterial strain was isolated from the soil of a Populus euphratica forest located in Xinjiang, China. The strain, designated strain HYL7-15(T), was subjected to a taxonomic analysis using a polyphasic approach. 16S rRNA gene sequence analyses indicated that the isolate belonged to the phylum Bacteroidetes and was related to the genus Pontibacter, with sequence similarities ranging from 93.1 to 95.0% with other species of the genus Pontibacter. Strain HYL7-15(T) contained MK-7 as the predominant menaquinone and its DNA G+C content was 44.9 mol%. The major cellular fatty acids of the novel strain were iso-C(15:0) (16.49%), iso-C(17:0) 3-OH (10.96%) and summed feature 4 (comprising anteiso-C(17:1) B and/or iso-C(17:1) I, 18.46%). The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG); diphosphatidylglycerol (DPG), three unknown aminophospholipids (APLs) and two unknown phospholipids (PLs) were also detected. On the basis of the evidence presented, it is concluded that strain HYL7-15(T) represents a novel species of the genus Pontibacter, for which the name Pontibacter populi sp. nov. is proposed. The type strain is HYL7-15(T) (=CCTCC AB 206239(T)=NRRL B-59488(T)).
一株革兰氏阴性、杆状、非运动和粉红色的细菌菌株从位于中国新疆的胡杨林中的土壤中分离得到。该菌株被命名为 HYL7-15(T)，采用多相分类法进行了分类学分析。16S rRNA 基因序列分析表明，该分离物属于拟杆菌门，与 Pontibacter 属有关，与 Pontibacter 属的其他种的序列相似性为 93.1%至 95.0%。HYL7-15(T) 株含有 MK-7 作为主要的menaquinone，其 DNA G+C 含量为 44.9 mol%。该新菌株的主要细胞脂肪酸为 iso-C(15:0)（16.49%）、iso-C(17:0) 3-OH（10.96%）和总和特征 4（包含 anteiso-C(17:1) B 和/或 iso-C(17:1) I，18.46%）。主要的极性脂为磷脂酰乙醇胺（PE）和磷脂酰甘油（PG）；还检测到二磷脂酰甘油（DPG）、三种未知的氨基磷脂（APLs）和两种未知的磷脂（PLs）。基于提出的证据，得出结论，菌株 HYL7-15(T) 代表 Pontibacter 属的一个新种，建议将其命名为 Pontibacter populi sp. nov.。模式菌株为 HYL7-15(T)（=CCTCC AB 206239(T)=NRRL B-59488(T)）。
