Jadhav Kiran, Gupta Nidhi, Ahmed Mujib Br
Department of Oral Pathology and Microbiology, Rural Dental College, Loni, India.
J Cytol. 2011 Jan;28(1):7-12. doi: 10.4103/0970-9371.76941.
Micronuclei in exfoliated oral epithelial cells have been shown in some studies to correlate with severity of this genotoxic damage. This severity can be measured in terms of grading of the lesions.
To correlate frequency of micronuclei (MN) in oral exfoliated cells in clinically diagnosed cases of oral squamous cell carcinoma (OSCC) followed by a histopathological grading.
The study subjects consisted of clinically diagnosed cases of OSCC. Healthy subjects without any tobacco consumption habits formed the control group. The cytosmears from both groups were stained with rapid Papanicolaou stain. MN were identified according to the criteria given by Countryman and Heddle with some modifications.
The frequency of MN was three to four times higher in patients with OSCC as compared to patients in the control group and the difference was found to be highly significant. In 75% cases, the cytological grade as determined by the frequency of micronuclei correlated with the histopathological grade and this observation was statistically significant.
MN can be a candidate to serve as a biomarker for prediction of the grade of OSCC.
一些研究表明，脱落口腔上皮细胞中的微核与这种基因毒性损伤的严重程度相关。这种严重程度可以通过病变分级来衡量。
对临床诊断的口腔鳞状细胞癌（OSCC）病例的口腔脱落细胞中的微核（MN）频率与组织病理学分级进行相关性研究。
研究对象为临床诊断的OSCC病例。无任何烟草消费习惯的健康受试者组成对照组。两组的细胞涂片均用快速巴氏染色法染色。MN根据Countryman和Heddle给出的标准并做了一些修改进行识别。
与对照组患者相比，OSCC患者的MN频率高出三到四倍，且差异具有高度显著性。在75%的病例中，由微核频率确定的细胞学分级与组织病理学分级相关，这一观察结果具有统计学意义。
MN可作为预测OSCC分级的生物标志物。
