Davis Joshua M, Maki James, Bahcall James K
Department of Graduate Endodontics, Marquette University, Milwaukee, WI 53233, USA.
J Endod. 2007 May;33(5):567-9. doi: 10.1016/j.joen.2007.01.015. Epub 2007 Mar 26.
The purpose of this in vitro study was to investigate the antimicrobial action of Dermacyn (Oculus Innovative Sciences, Petaluma, CA), BioPure MTAD (Dentsply Tulsa Dental, Johnson City, TN), 2% chlorhexidine (CHX; Ultradent, West Jordan, UT), and 5.25% sodium hypochlorite (NaOCl) against Enterococcus faecalis (American Type Culture Collection 4082). Eighteen Petri dishes of BHI agar were inoculated with E faecalis. Each Petri dish had five saturated paper disks placed. Four of the disks were saturated with a different test solution, and the last paper disk served as the control and was saturated with sterile distilled water. The plates were randomly distributed into two groups. Group one (n=9) was incubated aerobically and group 2 (n=9) was incubated anaerobically for 48 hours at 37 degrees C. The largest diameter of the zones of microbial inhibition was measured in millimeters and recorded. Statistical analysis was performed with repeated-measures analysis of variance. BioPure MTAD showed significantly (p<0.05) more zones of microbial inhibition than 5.25% NaOCl, 2% CHX, and Dermacyn. Sodium hypochlorite and CHX showed significantly (p<0.05) more zones of microbial inhibition than Dermacyn. The zone of inhibition between NaOCl and CHX was not significant (p>0.05). The control group showed no microbial inhibition.
本体外研究的目的是调查 Dermacyn（奥culus创新科学公司，加利福尼亚州佩塔卢马）、BioPure MTAD（登士柏塔尔萨牙科公司，田纳西州约翰逊城）、2%氯己定（CHX；Ultradent，犹他州西乔丹）和5.25%次氯酸钠（NaOCl）对粪肠球菌（美国典型培养物保藏中心4082）的抗菌作用。将18个装有脑心浸液琼脂的培养皿接种粪肠球菌。每个培养皿放置5个饱和纸片。其中4个纸片用不同的测试溶液饱和，最后一个纸片作为对照，用无菌蒸馏水饱和。平板被随机分为两组。第一组（n = 9）进行需氧培养，第二组（n = 9）进行厌氧培养，在37℃下培养48小时。测量并记录微生物抑制圈的最大直径（以毫米为单位）。采用重复测量方差分析进行统计分析。BioPure MTAD显示出比5.25% NaOCl、2% CHX和Dermacyn显著更多（p < 0.05）的微生物抑制圈。次氯酸钠和CHX显示出比Dermacyn显著更多（p < 0.05）的微生物抑制圈。NaOCl和CHX之间的抑制圈差异不显著（p > 0.05）。对照组未显示出微生物抑制。
